Human CCL20/MIP-3 alpha/MIP3A ELISA Pair Set(产品说明)
反应种属:Human
试剂对靶点:CCL20
试剂对应用:ELISA
试剂对保存条件:Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围:7.81-500
试剂对标记类型:HRP
包被抗体:1 mg/mL of mouse anti-Human CCL20/MIP-3 alpha/MIP3A monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10485-MM21)
检测抗体:0.2 mg/mL of mouse anti-Human CCL20/MIP-3 alpha/MIP3A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10485-MM07)
试剂灵敏度:The minimum detectable dose of Human CCL20/MIP-3 alpha/MIP3A was determined to be approximately 7.81 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理:The Human CCL20/MIP-3 alpha/MIP3A ELISA Pair Set is for the quantitative determination of Human CCL20/MIP-3 alpha/MIP3A.The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human CCL20/MIP-3 alpha/MIP3A coated on a 96-well plate. Standards and samples are added to the wells, and any Human CCL20/MIP-3 alpha/MIP3A present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human CCL20/MIP-3 alpha/MIP3A monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human CCL20/MIP-3 alpha/MIP3A present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限:7.81 pg/ml