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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 检测范围:
6.25 pg/ml
- 检测方法:
ELISA
- 应用:
ELISA
- 适应物种:
Human
- 标记物:
HRP
- 样本:
见说明书
- 规格:
5 Plates/15 Plates
| 规格: | 5 Plates | 产品价格: | ¥2299.0 |
|---|---|---|---|
| 规格: | 15 Plates | 产品价格: | ¥5599.0 |
反应种属:Human
试剂对靶点:LTA
试剂对应用:ELISA
试剂对保存条件:Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围:6.25-400
试剂对标记类型:HRP
包被抗体:0.4 mg/mL of mouse anti-Human TNF-beta / TNFSF1 / Lymphotoxin alpha monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10270-MM02)
检测抗体:0.2 mg/mL of rabbit anti-Human TNF-beta / TNFSF1 / Lymphotoxin alpha monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10270-R074)
试剂灵敏度:The minimum detectable dose of Human TNF-beta / TNFSF1 / Lymphotoxin alpha was determined to be approximately 6.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理:The Human TNF-beta / TNFSF1 / Lymphotoxin alpha ELISA Pair Set is for the quantitative determination of Human TNF-beta / TNFSF1 / Lymphotoxin alpha. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human TNF-beta / TNFSF1 / Lymphotoxin alpha coated on a 96-well plate. Standards and samples are added to the wells, and any Human TNF-beta / TNFSF1 / Lymphotoxin alpha present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human TNF-beta / TNFSF1 / Lymphotoxin alpha monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human TNF-beta / TNFSF1 / Lymphotoxin alpha present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限:6.25 pg/ml
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xungufeng820 各位大侠: 小弟是新手,师兄们也没做过的,不知western-blot测NF-KB、FLT3是用总蛋白提取试剂盒吗?急盼回复!万分感谢! snowxyh 我用过总蛋白提取试剂盒提蛋白,之后western-blot检测NF-KB,但是感觉这样得到的蛋白浓度很稀,需要比较大的上样量才能检测出来,个人感觉,还是用传统的细胞匀浆裂解,离心提取上清,这样得到的蛋白浓度大,做试验结果好
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