Sino Biological Human CD44 ELISA配对试剂盒 人源-ELISA检测 cd44试剂盒

Human CD44 ELISA Pair Set（产品说明）
反应种属：Human
试剂对靶点：CD44
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：125-8000
试剂对标记类型：HRP
包被抗体：0.2 mg/mL of mouse anti-CD44 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 12211-MM01)
检测抗体：0.25 mg/mL mouse anti-CD44 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 12211-MM10)
试剂灵敏度：The minimum detectable dose of Human CD44 was determined to be approximately 125 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Human CD44 ELISA Pair Set is for the quantitative determination of Human CD44. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD44 coated on a 96-well plate. Standards and samples are added to the wells, and any CD44 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-CD44 monoclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD44 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：125 pg/ml