- ￥2299 - 5599
- Sino Biological
- 15.63 pg/ml
- 北京
- SEK10008
- 2025年08月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 检测范围：
15.63 pg/ml
- 检测方法：
ELISA
- 应用：
ELISA
- 适应物种：
Human
- 标记物：
HRP
- 样本：
见说明书
- 规格：
5 Plates/15 Plates
| 规格： | 5 Plates | 产品价格： | ￥2299.0 |
|---|---|---|---|
| 规格： | 15 Plates | 产品价格： | ￥5599.0 |
反应种属：Human
试剂对靶点：VEGFA
试剂对应用：ELISA
试剂对保存条件：Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
试剂对线性范围：15.63-1000
试剂对标记类型：HRP
包被抗体：1 mg/mL of rabbit anti-Human VEGF/VEGFA/VEGF121 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 μg/mL in PBS before coating. (Catalog: # 10008-R145)
检测抗体：0.2 mg/mL of rabbit anti-Human VEGF/VEGFA/VEGF121 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.15 μg/mL in Dilution Buffer before use. (Catalog: # 10008-R064)
试剂灵敏度：The minimum detectable dose of Human VEGF/VEGFA/VEGF121 was determined to be approximately 15.63 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
试剂对检测原理：The Human VEGF/VEGFA/VEGF121 ELISA Pair Set is for the quantitative determination of Human VEGF/VEGFA/VEGF121. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human VEGF/VEGFA/VEGF121 coated on a 96-well plate. Standards and samples are added to the wells, and any Human VEGF/VEGFA/VEGF121 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human VEGF/VEGFA/VEGF121 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human VEGF/VEGFA/VEGF121 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
试剂对检测限：15.63 pg/ml
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Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures
to determine agonist-induced mucin secretion from goblet cells in human bronchial epithelial (HBE) cell cultures. It begins with primary epithelial cell culture, offers methods for purifying MUC5AC and MUC5B mucins for standards, and describes five different
Endothelial Cell Systems - Instructions For Use
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