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- Sino Biological
- 北京
- VG40283-ACR
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
3个月
- 英文名：
Hepatitis C virus NS4B Gene ORF cDNA clone expression plasmid, C-OFPSpark / RFP tag
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1 Unit
基因靶点：HCV-NS4B
反应种属：HCV
应用说明：Stable or Transient mammalian expression
cDNA描述：Full length Clone DNA of Hepatitis C virus (HCV) (serotype 1a, isolate H77) NS4B with C terminal OFPSpark / RFP tag.
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文献和实验Use of the Vaccinia Virus/T7 Expression System for Studying HCV Protein Processing
polyprotein appears to be cleaved at multiple sites to produce at least 10 structural and nonstructural (NS) proteins (9 ). The order and nomenclature of these cleavage products for the HCV-H strain are NH2 -C-El-E2-p7-NS2-NS3-NS4A-NS4B-NS5A-NS5B-COOH
Methods for the Study of Sequence-Specific Binding of Proteins to the HCV RNA Genome
The appropriate formation of specific RNA-protein complexes regulates the normal synthesis, trafficking, and metabolism of intracellular RNA. For RNA viruses, these interactions are essential for replication and translation of the viral
expression (a confirmative experiment on the expression of the green fluorescent protein (GFP)) in the HEK 293 and NIH-3T3 cells is indicated in Fig. 1A). We expected that the hypothermic temperature also suppresses the mammalian RNAi to support
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