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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
3个月
- 英文名:
Mouse SERPINA1B Gene ORF cDNA clone expression plasmid, C-GFPSpark tag
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1 Unit
| 货号: | 表达载体: |
| MG50680-ACG | pCMV3-C-GFPSpark |
| MG50680-CF | pCMV3-C-FLAG |
| MG50680-CH | pCMV3-C-His |
| MG50680-CM | pCMV3-C-Myc |
| MG50680-CY | pCMV3-C-HA |
| MG50680-NF | pCMV3-SP-N-FLAG |
| MG50680-NH | pCMV3-SP-N-His |
| MG50680-NM | pCMV3-SP-N-Myc |
| MG50680-NY | pCMV3-SP-N-HA |
| MG50680-UT | pCMV3-untagged |
| MG50680-M | pMD18-T Simple Vector |
| MG50680-ACR | pCMV3-C-OFPSpark |
| MG50680-ACGLN | pLV-C-GFPSpark |
Mouse SERPINA1B Gene ORF cDNA clone expression plasmid, C-GFPSpark tag产品信息
基因靶点:SERPINA1B
反应种属:Mouse
应用说明:Stable or Transient mammalian expression
cDNA描述:Full length Clone DNA of Mouse serine (or cysteine) preptidase inhibitor, clade A, member 1b with C terminal GFPSpark tag.
| 货号: | MG50680-ACG |
| 产品名称: | Mouse SERPINA1B Gene ORF cDNA clone expression plasmid, C-GFPSpark tag |
| NCBI 参考序列号: | NM_009244.4 |
| 序列长度: | 1242 |
| 表达载体: | pCMV3-C-GFPSpark |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) preptidase inhibitor, clade A, member 1b with C terminal GFPSpark tag. |
| 货号: | MG50680-CF |
| 产品名称: | Mouse SERPINA1B Gene ORF cDNA clone expression plasmid, C-Flag tag |
| NCBI 参考序列号: | NM_009244.4 |
| 序列长度: | 1242 |
| 表达载体: | pCMV3-C-FLAG |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) preptidase inhibitor, clade A, member 1b with C terminal Flag tag. |
| 货号: | MG50680-CH |
| 产品名称: | Mouse SERPINA1B Gene ORF cDNA clone expression plasmid, C-His tag |
| NCBI 参考序列号: | NM_009244.4 |
| 序列长度: | 1287 |
| 表达载体: | pCMV3-C-His |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) preptidase inhibitor, clade A, member 1b with C terminal His tag. |
| 货号: | MG50680-CM |
| 产品名称: | Mouse SERPINA1B Gene ORF cDNA clone expression plasmid, C-Myc tag |
| NCBI 参考序列号: | NM_009244.4 |
| 序列长度: | 1242 |
| 表达载体: | pCMV3-C-Myc |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) preptidase inhibitor, clade A, member 1b with C terminal Myc tag. |
| 货号: | MG50680-CY |
| 产品名称: | Mouse SERPINA1B Gene ORF cDNA clone expression plasmid, C-HA tag |
| NCBI 参考序列号: | NM_009244.4 |
| 序列长度: | 1242 |
| 表达载体: | pCMV3-C-HA |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) preptidase inhibitor, clade A, member 1b with C terminal HA tag. |
| 货号: | MG50680-NF |
| 产品名称: | Mouse SERPINA1B Gene ORF cDNA clone expression plasmid, N-Flag tag |
| NCBI 参考序列号: | NM_009244.4 |
| 序列长度: | 1242 |
| 表达载体: | pCMV3-SP-N-FLAG |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) preptidase inhibitor, clade A, member 1b with N terminal Flag tag. |
| 货号: | MG50680-NH |
| 产品名称: | Mouse SERPINA1B Gene ORF cDNA clone expression plasmid, N-His tag |
| NCBI 参考序列号: | NM_009244.4 |
| 序列长度: | 1242 |
| 表达载体: | pCMV3-SP-N-His |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) preptidase inhibitor, clade A, member 1b with N terminal His tag. |
| 货号: | MG50680-NM |
| 产品名称: | Mouse SERPINA1B Gene ORF cDNA clone expression plasmid, N-Myc tag |
| NCBI 参考序列号: | NM_009244.4 |
| 序列长度: | 1242 |
| 表达载体: | pCMV3-SP-N-Myc |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) preptidase inhibitor, clade A, member 1b with N terminal Myc tag. |
| 货号: | MG50680-NY |
| 产品名称: | Mouse SERPINA1B Gene ORF cDNA clone expression plasmid, N-HA tag |
| NCBI 参考序列号: | NM_009244.4 |
| 序列长度: | 1242 |
| 表达载体: | pCMV3-SP-N-HA |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) preptidase inhibitor, clade A, member 1b with N terminal HA tag. |
| 货号: | MG50680-UT |
| 产品名称: | Mouse SERPINA1B Gene ORF cDNA clone expression plasmid |
| NCBI 参考序列号: | NM_009244.4 |
| 序列长度: | 1242 |
| 表达载体: | pCMV3-untagged |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) preptidase inhibitor, clade A, member 1b. |
| 货号: | MG50680-M |
| 产品名称: | Mouse SERPINA1B Gene ORF cDNA clone in cloning vector |
| NCBI 参考序列号: | NM_009244.4 |
| 序列长度: | 1242 |
| 表达载体: | pMD18-T Simple Vector |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) preptidase inhibitor, clade A, member 1b. |
| 货号: | MG50680-ACR |
| 产品名称: | Mouse SERPINA1B Gene ORF cDNA clone expression plasmid, C-OFPSpark tag |
| NCBI 参考序列号: | NM_009244.4 |
| 序列长度: | 1242 |
| 表达载体: | pCMV3-C-OFPSpark |
| cDNA描述: | Full length Clone DNA of Mouse serine (or cysteine) preptidase inhibitor, clade A, member 1b with C terminal OFPSpark / RFP tag. |
| 货号: | MG50680-ACGLN |
| 产品名称: | Mouse SERPINA1B Gene Lentiviral ORF cDNA expression plasmid, C-GFPSpark tag |
| NCBI 参考序列号: | NM_009244.4 |
| 序列长度: | 1242 |
| 表达载体: | pLV-C-GFPSpark |
| cDNA描述: | Full length Clone DNA of Mus musculus serine (or cysteine) preptidase inhibitor, clade A, member 1b. |
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文献和实验Using the DFCI Gene Index Databases for Biological Discovery
. Genet. 10:369‐371. Cariaso, M., Folta, P., Wagner, M., Kuczmarski, T., and Lennon, G. 1999. IMAGEne I: Clustering and ranking of I.M.A.G.E. cDNA clones corresponding to known
我的题目是鸡Mx基因的克隆,我的策略是先根据该基因家族(dynamin family)的氨基酸保守区设计简并引物克隆出该基因cDNA的部分片段,然后根据该片段序列设计基因特异性引物,利用RACE技术得到cDNA的3‘和5’末端,然后根据末端序列设计引物扩增出该基因全长cDNA. 我用简并引物从鸡cDNA中扩增出一个片段,约250bp, 测序后拿来BLAST时却发现,与之同源性最高的并不是其它的Mx基因,而是人的某一个基因,比对的结果中与该序列同源性较高的有人及鼠Mx基因,但是同源性并不
的位置及功能位点,以及标记已知的序列模式等过程。在此过程中,确认一段DNA序列是一个基因需要有多个证据的支持: ) E/ C0 ^ @' o% B0 \* [# B! V2 W1、一般而言,在重复片段频繁出现的区域里,基因编码区和调控区不太可能出现;; p/ u- p) P. N5 y( m) P 2、如果某段DNA片段的假想产物与某个已知的蛋白质或其它基因的产物具有较高序列相似性的话,那么这个DNA片段就非常可能属于外显子片段
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