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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
3个月
- 英文名:
Mouse RNGTT Gene ORF cDNA clone expression plasmid, C-GFPSpark tag
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1 Unit
| 货号: | 表达载体: |
| MG53041-ACG | pCMV3-C-GFPSpark |
| MG53041-ANG | pCMV3-N-GFPSpark |
| MG53041-CF | pCMV3-C-FLAG |
| MG53041-CH | pCMV3-C-His |
| MG53041-CM | pCMV3-C-Myc |
| MG53041-CY | pCMV3-C-HA |
| MG53041-G | PGEM-T Vector |
| MG53041-NF | pCMV3-N-FLAG |
| MG53041-NH | pCMV3-N-His |
| MG53041-NM | pCMV3-N-Myc |
| MG53041-NY | pCMV3-N-HA |
| MG53041-UT | pCMV3-untagged |
| MG53041-ACR | pCMV3-C-OFPSpark |
| MG53041-ANR | pCMV3-N-OFPSpark |
| MG53041-ACGLN | pLV-C-GFPSpark |
Mouse RNGTT Gene ORF cDNA clone expression plasmid, C-GFPSpark tag产品信息
基因靶点:RNGTT
反应种属:Mouse
应用说明:Stable or Transient mammalian expression
cDNA描述:Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase with C terminal GFPSpark tag.
| 货号: | MG53041-ACG |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone expression plasmid, C-GFPSpark tag |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pCMV3-C-GFPSpark |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase with C terminal GFPSpark tag. |
| 货号: | MG53041-ANG |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone expression plasmid, N-GFPSpark tag |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pCMV3-N-GFPSpark |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase with N terminal GFPSpark tag. |
| 货号: | MG53041-CF |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone expression plasmid, C-Flag tag |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pCMV3-C-FLAG |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase with C terminal Flag tag. |
| 货号: | MG53041-CH |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone expression plasmid, C-His tag |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pCMV3-C-His |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase with C terminal His tag. |
| 货号: | MG53041-CM |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone expression plasmid, C-Myc tag |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pCMV3-C-Myc |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase with C terminal Myc tag. |
| 货号: | MG53041-CY |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone expression plasmid, C-HA tag |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pCMV3-C-HA |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase with C terminal HA tag. |
| 货号: | MG53041-G |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone in cloning vector |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | PGEM-T Vector |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase. |
| 货号: | MG53041-NF |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone expression plasmid, N-Flag tag |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pCMV3-N-FLAG |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase with N terminal Flag tag. |
| 货号: | MG53041-NH |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone expression plasmid, N-His tag |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pCMV3-N-His |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase with N terminal His tag. |
| 货号: | MG53041-NM |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone expression plasmid, N-Myc tag |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pCMV3-N-Myc |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase with N terminal Myc tag. |
| 货号: | MG53041-NY |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone expression plasmid, N-HA tag |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pCMV3-N-HA |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase with N terminal HA tag. |
| 货号: | MG53041-UT |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone expression plasmid |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pCMV3-untagged |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase. |
| 货号: | MG53041-ACR |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone expression plasmid, C-OFPSpark tag |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pCMV3-C-OFPSpark |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase with C terminal OFPSpark / RFP tag. |
| 货号: | MG53041-ANR |
| 产品名称: | Mouse RNGTT Gene ORF cDNA clone expression plasmid, N-OFPSpark tag |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pCMV3-N-OFPSpark |
| cDNA描述: | Full length Clone DNA of Mouse RNA guanylyltransferase and 5'-phosphatase with N terminal OFPSpark / RFP tag. |
| 货号: | MG53041-ACGLN |
| 产品名称: | Mouse RNGTT Gene Lentiviral ORF cDNA expression plasmid, C-GFPSpark tag |
| NCBI 参考序列号: | NM_011884.2 |
| 序列长度: | 1794 |
| 表达载体: | pLV-C-GFPSpark |
| cDNA描述: | Full length Clone DNA of Mus musculus RNA guanylyltransferase and 5'-phosphatase. |
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文献和实验。我也碰到过类似的情况,直接用载体转染筛选,得到的稳定表达只有40-50%的细胞带GFP,而筛选单克隆时,从单克隆细胞得到的细胞群也是相似的情况,大概只有50%左右GFP阳性。我个人觉得,质粒直接转染本身整合率就很低,而且整合也不稳定,所以在每一次细胞分裂时就有可能丢失,或产生错误的整合使得GFP基因不完整插入到基因组中。后来,用逆转录病毒感染后,再用G418筛选,就得到100% 的GPF了,因为逆转录病毒带有整合酶和重复长末端序列,这时的整合应该是不是破坏GFP的稳定性。这仅是我个人的猜测
【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
检测GFP、EGFP、YFP、EYFP、CFP的GFP抗体GFP是绿色萤光蛋白(Green Fluorescent Protein)的简称,由238个氨基酸残基组成。GFP蛋白质最早是由下村脩等人在1962年在一种学名Aequorea victoria的水母中发现。其基因所产生的蛋白质,在蓝色波长范围的光线激发下,会发出绿色萤光。GFP或其突变体EGFP标签系统等被广泛用于基因表达效率的检测,以及和目的蛋白融合表达用于检测目的蛋白的表达和分布。我们都知道GFP抗体能应用于GFP的表达检测、纯化
以反转录产物为模板,克隆引物B1,B2为引物,利用rTaq酶进行扩增目的基因,用于后续连入克隆载体的操作,然后再以表达引物EB1,EB2为引物,利用Pfu DNA聚合酶进行扩增目的基因,用于后续连入表达载体的操作。 94 ℃ 3 min 94 ℃ 45Sec 30 cycles: 57 ℃ 45Sec 72 ℃ 45Sec 72 ℃ 5min
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