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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human PIG3 / TP53I3 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1 Unit
基因靶点：TP53I3
反应种属：Human
应用说明：Stable or Transient mammalian expression
cDNA描述：Full length Clone DNA of Human tumor protein p53 inducible protein 3 with C terminal OFPSpark / RFP tag.
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文献和实验Co-Expression of human DNA primase p49-p58 subunits
.Note: You don't have to shake (the Fanning lab simply transfers their flasks to a RT H2O bath),but I found that it does improve expression levels. 6.Harvest by centrifugation,5000 rpm,10 min.,4℃.Freeze pellets at -80℃. Purification of p49-p58 human
Strategies to Optimize Protein Expression in E. coli
Burgess‐Brown, N.A., Sharma, S., Sobott, F., Loenarz, C., Oppermann, U., and Gileadi, O. 2008. Codon optimization can improve expression of human genes in Escherichia coli: A multi‐gene study. Protein Expr. Purif. 59:94‐102
primers and ligated into a mammalian expression vector under the control of a CMV promoter. Identification of a plasmid possessing the open reading frame of the paraoxonase-1 gene was carried out by PCR analysis using gene specific primers. Confirmation
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