- ￥1890
- Sino Biological
- 北京
- HG12036-NH
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human COX-2/PTGS2 Gene ORF cDNA clone expression plasmid, N-His tag
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1 Unit
基因靶点：PTGS2
反应种属：Human
应用说明：Stable or Transient mammalian expression
cDNA描述：Full length Clone DNA of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) with N terminal His tag.
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文献和实验2, Lee JY, et al. N-AS-triggered SPMs are direct regulators of microglia in a model of Alzheimer's disease.Nature communications, PubMed ID: 32398649
Expression of Recombinant Proteins with Uniform N-Termini
that recombinant proteins produced in this expression system contain homogeneous, unprocessed N-termini.
The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
on engineered aminopeptidases. Using human tumor necrosis factor α as a model protein, we describe here the steps involved in the removal of a His-tag using TAGZyme. The tag used (UZ-HT15) has been optimized for expression in Escherichia coli and for TAGZyme
Co-Expression of human DNA primase p49-p58 subunits
.Note: You don't have to shake (the Fanning lab simply transfers their flasks to a RT H2O bath),but I found that it does improve expression levels. 6.Harvest by centrifugation,5000 rpm,10 min.,4℃.Freeze pellets at -80℃. Purification of p49-p58 human
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