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- Sino Biological
- 北京
- HG12107-CF
- 2025年08月12日
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- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human PPARGC1A/PGC1 Gene ORF cDNA clone expression plasmid, C-Flag tag
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1 Unit
基因靶点：PPARGC1A
反应种属：Human
应用说明：Stable or Transient mammalian expression
cDNA描述：Full length Clone DNA of Human peroxisome proliferator-activated receptor gamma, coactivator 1 alpha with C terminal Flag tag.
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文献和实验Co-Expression of human DNA primase p49-p58 subunits
.Note: You don't have to shake (the Fanning lab simply transfers their flasks to a RT H2O bath),but I found that it does improve expression levels. 6.Harvest by centrifugation,5000 rpm,10 min.,4℃.Freeze pellets at -80℃. Purification of p49-p58 human
3X Flag Peptide) to each pelleted bead/complex, incubate 30-60 minutes at 4°C with gentle shaking;Centrifuge the resin for 30 seconds at 10,000 RPM, transfer the supernatants (which should now contain the ChIP/Flag eluted complexes) to fresh tubes
Purification of Human Multiprotein Complexes using OneSTrEP Technology
- or C-terminal of YPI, clones can be isolated in 96 wells following 2 weeks of G418 selection (note 1 ). We recommend to isolate several clones and characterize them by a few simple criteria: Western blotting to check expression level
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