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- Sino Biological
- 北京
- HG16319-NF
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human SPIDR Gene ORF cDNA clone expression plasmid, N-Flag tag
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1 Unit
基因靶点：SPIDR
反应种属：Human
应用说明：Stable or Transient mammalian expression
cDNA描述：Full length Clone DNA of Human scaffolding protein involved in DNA repair with N terminal Flag tag.
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文献和实验Co-Expression of human DNA primase p49-p58 subunits
.Note: You don't have to shake (the Fanning lab simply transfers their flasks to a RT H2O bath),but I found that it does improve expression levels. 6.Harvest by centrifugation,5000 rpm,10 min.,4℃.Freeze pellets at -80℃. Purification of p49-p58 human
Purification of Human Multiprotein Complexes using OneSTrEP Technology
Introduction Here we describe a strategy for isolation of multiprotein complexes from human HeLa S3 cells in a scale and purity optimized for characterization by mass spectrometry. For this purpose, we use stably expressed
Strategies to Optimize Protein Expression in E. coli
., Wermke, N., Scheuermann, T., Ebert, L., LaBaer, J., and Korn, B. 2007. A systematic approach for testing expression of human full‐length proteins in cell‐free expression systems. BMC Biotechnol. 7:64.
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