- ¥980
- frdbio
- TAG0050H
- 武汉
- 2025年12月23日
- WB,ELISA
- 小鼠
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- 详细信息
- 文献和实验
- 技术资料
- 浓度：
0.5-1mg/ml
- 应用范围：
WB,ELISA
- 宿主：
小鼠
- 标记物：
HRP标记
- 克隆性：
单克隆
- 保存条件：
-20℃
- 形态：
液体
- 规格：
50ul
Catalog No.: TAG0050H
Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store: at -20°C. Do not aliquot the antibody.
Recommended dilutions:
ELISA:1:1000-10000 WB: 1:500-5000
Optimal dilutions should be determined by the end user.
Background: The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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