- ¥980
- frdbio
- TAG0030H
- 武汉
- 2025年12月24日
- WB,ELISA
- 鼠
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- 详细信息
- 文献和实验
- 技术资料
- 浓度：
0.5-1mg/ml
- 应用范围：
WB,ELISA
- 宿主：
鼠
- 标记物：
HRP
- 克隆性：
单克隆
- 保存条件：
-20℃
- 形态：
液体
- 规格：
50ul
Catalog No: TAG0030H
Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.
Recommended dilutions:
ELISA:1:1000-10000 WB: 1:500-5000
Optimal dilutions should be determined by the end user.
Background: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.HA tag antibody is a highly sensitive and affinity monoclonal antibody applicable to HA-tagged fusion protein detection.
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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