- ¥980
- frdbio
- RAB0100H
- 2025年12月24日
- WB,ELISA
- 鼠
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- 详细信息
- 文献和实验
- 技术资料
- 浓度：
0.5-1mg/ml
- 应用范围：
WB,ELISA
- 宿主：
鼠
- 标记物：
HRP
- 克隆性：
单克隆
- 形态：
液体
- 规格：
50ul
Catalog No.: RAB0100H
Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Store at -20°C. Do not aliquot the antibody.
Molecular Weight:43 KD
Recommended dilutions:
ELISA:1:1000-10000 WB: 1:500-2000
Optimal dilutions should be determined by the end user.
Background: β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.
Note: This product is for research use only,not for diagnostic or therapeutic use.
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文献和实验常用的β-actin 引物序列 human actin f ctc cat cct ggc ctc gct gt human actin r gct gtc acc ttc acc gtt cc product size:268 rabbit actin r agt gcg acg tgg aca tcc g rabbit actin f tgg ctc taa cag tcc gcc tag product size:295 mouse actin r cgt
human actin f ctc cat cct ggc ctc gct gt human actin r gct gtc acc ttc acc gtt cc product size:268 rabbit actin r agt gcg acg tgg aca tcc g rabbit actin f tgg ctc taa cag tcc gcc ta product size:295 mouse actin r cgt tga cat ccg taa aga
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
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