- ¥980
- frdbio
- TAG0010H
- 2025年12月24日
- WB,ELISA
- 鼠
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- 详细信息
- 文献和实验
- 技术资料
- 浓度：
0.5-1mg/ml
- 应用范围：
WB,ELISA
- 宿主：
鼠
- 标记物：
HRP
- 克隆性：
单克隆
- 保存条件：
-20℃
- 形态：
液体
- 规格：
50ul
Catalog No.: TAG0010H
Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C. Do not aliquot the antibody.
Recommended dilutions:
ELISA: 1:1,000-100000 WB: 1:1,000-20000
Optimal dilutions should be determined by the end user.
Background:A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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