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35
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详见说明书
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| 产品名称 | 英文名称 | 价格 |
| human Transferrin protein多少钱 | 电询 |
别名:Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesized in the liver and consists of two dom
产品类型:Native protein
宿主:Purified from Human plasma
应用:Western blotting,ELISA,etc.
肽:Full length native Human Transferrin
蛋白纯度:>90%(SDS-PAGE)
关联:Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesized in the liver and consists of two dom
缓冲液:PBS
形态:Liquid
贮存稳定性:Aliquot and store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
human Transferrin protein多少钱不同表达形式的蛋白应采用的的策略:
1、融合型表达重组蛋白,一般胞内可溶性的,拟选用亲和层析进行纯化;
2、测定范围:含氮量0.05%-90%; 分泌型表达的重组蛋白,通常体积大、浓度低,因此应在纯化之前采用沉淀或超滤等方法先进行浓缩处理;
3、包涵体型表达的重组蛋白,应先离心回收包涵体;
4、表达在细胞膜和细胞壁之间的间隙中的蛋白质,应用低浓度的溶菌酶,然后再用渗透压休克法释放重组蛋白。
不同纯化方法之间的平衡:
1、分辨率:由选择的方法和层析介质生成窄峰的能力来完成。当杂质和目的蛋白性质相似是,在纯化的zui后阶段分辨率是选择层析方法的重要考量标准。
2、处理量:指在纯化过程中目标蛋白的上样体积和浓度等。
3、速度:在初纯化中是重要因素,此时杂质如蛋白酶必须尽快去除。
4、回收率:随着纯化的进行渐趋重要,因为纯化产物的价值在增加。
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