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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of MPS1 expressed in E. coli.
- 亚型:
Mouse IgG1
- 形态:
Each vial contains 0.1 ml IgG in PBS pH7.4 with 0.03% sodium azide. Antibody was purified by Protein-G affinity chromatography.
- 保存条件:
Store at -20°C
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 适应物种:
H
- 保质期:
1-2年
- 抗原来源:
Purified recombinant fragment of MPS1 expressed in E. coli.
- 目录编号:
252892
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
IF
- 浓度:
见详细说明信息
- 靶点:
MPS1 (7E3)
- 抗体英文名:
MPS1 (7E3) Antibody
- 抗体名:
MPS1 (7E3) Antibody
- 规格:
0.1 ml
MPS1 (7E3) Antibody产品详细介绍:
| 产品名称: | MPS1 (7E3) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 252892 |
| 规格: | 0.1 ml |
| 产品描述: | MPS1 (Metallopan-stimulin 1) is a ribosomal protein. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. MPS1 is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. MSP1 has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG1 |
| 免疫原: | Purified recombinant fragment of MPS1 expressed in E. coli. |
| 适用物种: | H |
| 应用范围: | IF |
| 应用说明: | IF: 1:200-1:1000; |
| 别名: | 40S ribosomal protein S27; Metallopan-stimulin 1; MPS-1; RPS27 |
| 形态: | Each vial contains 0.1 ml IgG in PBS pH7.4 with 0.03% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件: | Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
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文献和实验人雌三醇 ( E3 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E3 单抗包被于酶标板上,标准品和样品中的 E3与单抗结合,加入生物素化的抗人 E3 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测
的单个Lys残基被多个泛素分子标记则称为多聚泛素化。 简而言之,也就是在E1、E2、E3三类酶的顺序催化下,泛素被以特异性的方式连接到靶标蛋白质或靶标蛋白质上已连接的泛素链上,其中E3泛素化连接酶决定靶蛋白的特异性识别。蛋白质泛素化的结果是使得被标记的蛋白质被蛋白酶分解为较小的多肽、氨基酸以及可以重复使用的泛素。 泛素化抗体的应用 自泛素-蛋白酶体系统在20世纪80年代初被发现以来,与其相关的研究成果层出不穷。泛素对细胞的生命活动不可或缺,但由于泛素化的复杂
Studies of the Ubiquitin Proteasome System
phosphatase inhibitors and 50 µM MG132 (or lactacystin) Anti‐E3 or anti‐substrate antibody Nondenaturing Triton X‐100 wash buffer (see recipe ) Tris‐buffered saline (TBS
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