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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
cAMP conjugated to KLH.
- 亚型:
Mouse IgG1
- 形态:
Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide.
- 保存条件:
Store at -20°C
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 保质期:
1-2年
- 抗原来源:
cAMP conjugated to KLH.
- 目录编号:
252762
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
E
- 浓度:
见详细说明信息
- 靶点:
cAMP (9H4C)
- 抗体英文名:
cAMP (9H4C) Antibody
- 抗体名:
cAMP (9H4C) Antibody
- 规格:
0.1 ml
cAMP (9H4C) Antibody产品详细介绍:
| 产品名称: | cAMP (9H4C) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 252762 |
| 规格: | 0.1 ml |
| 产品描述: | cAMP (Cyclic adenosine monophosphate) is a second messenger important in many biological processes. cAMP is derived from adenosine triphosphate (ATP) and used for intracellular signal transduction in many different organisms. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG1 |
| 免疫原: | cAMP conjugated to KLH. |
| 适用物种: | |
| 应用范围: | E |
| 应用说明: | E: 1:10,000-1:50,000 |
| 别名: | Cyclic amp; cAMP; cyclic AMP; 3'-5'-cyclic adenosine monophosphate |
| 形态: | Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide. |
| 保存条件: | Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
ABBIOTEC产品提供质量保证,提供完善的售后和技术支持,ABBIOTEC产品正品包装:
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文献和实验of soluble cAMP in bacterial extracts to compete with binding of an alkaline phosphatase (AP)-conjugated anti-cAMP antibody to immobilized cAMP. The surface-bound, immobilized anti-cAMP-AP is inversely correlated to the concentration of cAMP in the extract
In Vivo Actin Polymerization ( cAMP)
a. Wash cells with DB and resuspend them to 2 X 107 / ml inDB b. Shake cells at 120 rpm for 1 hour c. Pulse cells with 100 nM cAMP for 4 hours d. Basolate cells with 3 mM caffeine for 15-30 min e. Spin cells down, add DB + 3 mM
) b :取5μl b-cAMP加到25μl检测缓冲液中(b液) c :取5μl a液加到615μl检测缓冲液中,再加入5μl b液,轻轻混匀,室温至少孵育15min,形成检测复合物,顺序很重要,别错了,先稀释EU,后加b-cAMP。 ④标准品测定(在白色384孔板中反应,每个样品均为复孔,反应体积24μl); l 加入6μlcAMP标准品(刺激缓冲液配制); l 加入6μlAlexafluor标记的cAMP抗体工作
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