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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of PRAK expressed in E. coli.
- 亚型:
Mouse IgG1
- 形态:
Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide.
- 保存条件:
Store at -20°C
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 适应物种:
H
- 保质期:
1-2年
- 抗原来源:
Purified recombinant fragment of PRAK expressed in E. coli.
- 目录编号:
252720
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
WB, IHC
- 浓度:
见详细说明信息
- 靶点:
PRAK (7H10)
- 抗体英文名:
PRAK (7H10) Antibody
- 抗体名:
PRAK (7H10) Antibody
- 规格:
0.1 ml
PRAK (7H10) Antibody产品详细介绍:
| 产品名称: | PRAK (7H10) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 252720 |
| 规格: | 0.1 ml |
| 产品描述: | PRAK (p38-regulated /activated kinase) is a ubiquitously expressed serine/threonine kinase regulated by p38α and p38β MAP kinases. Activated JNK, p38γ or p38δ are unable to induce phosphorylation of PRAK in vitro. Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines. Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45% sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro. Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG1 |
| 免疫原: | Purified recombinant fragment of PRAK expressed in E. coli. |
| 适用物种: | H |
| 应用范围: | WB, IHC |
| 应用说明: | WB: 1:500-1:2000; IHC: 1:200-1:1000; |
| 别名: | MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MK-5; MK5; p38-regulated/activated protein kinase; PRAK; MAPKAPK5; |
| 形态: | Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide. |
| 保存条件: | Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
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文献和实验:化学发光法(曝光5分钟)。3、WB的背景过高,信号过低,看不到特异性条带--SignalBoostTM可以大大提高信噪比,使目的条带完美呈现: 一抗:anti-p-PRAK,分别用5%脱脂奶粉PBST溶液(图A)和Solution 1(图B)按照1:1000比例稀释。 二抗:用5%脱脂奶粉PBST溶液(图A)和Solution 2(图B)按照1:2000比例稀释。 4、SignalBoostTM可以使信号更强,减少抗体用量,节约成本--花更少的钱,得到更好的结果: 一抗:anti
线粒体荧光探针大全:TMRM,Mitotracker,JC-1
of the OxPhos Complex may also be used for immunohistochemical analysis. Image analysis of the antibody's staining pattern can reveal the relative expression and localization of a subunit. This approach has been particularly useful for studying OxPhos subunit
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