- ￥3000 - 3999
- Abbiotec
- 美国
- 252714
- 2025年11月20日
- WB, IHC
- Mouse
- H
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified recombinant fragment of human TIP60 expressed in E. coli.
- 亚型：
Mouse IgG2b
- 形态：
Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide.
- 保存条件：
Store at -20°C
- 克隆性：
单克隆
- 标记物：
见下方详细说明
- 适应物种：
H
- 保质期：
1-2年
- 抗原来源：
Purified recombinant fragment of human TIP60 expressed in E. coli.
- 目录编号：
252714
- 级别：
超纯
- 库存：
大量
- 供应商：
Abbiotec
- 宿主：
Mouse
- 应用范围：
WB, IHC
- 浓度：
见详细说明信息
- 靶点：
TIP60 (3F9)
- 抗体英文名：
TIP60 (3F9) Antibody
- 抗体名：
TIP60 (3F9) Antibody
- 规格：
0.1 ml
TIP60 (3F9) Antibody产品详细介绍：
| 产品名称： | TIP60 (3F9) Antibody |
| 说明书： | 【点击查看详细说明及参考图片】 |
| 货号： | 252714 |
| 规格： | 0.1 ml |
| 产品描述： | HTATIP (HIV-1 Tat interacting protein TIP60) is ~60kDa protein. It belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4) is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure. There are five major variants of histone H2A: canonical H2A (most abundant), H2A.X, MacroH2A, H2ABbd and H2A.Z. Histone H2A.Z, the most conserved variant across species, functions as both a positive and negative regulator of transcription and is important for chromosome stability. Several homologous protein complexes, such as SWR-C, TIP60 and SRCAP (mammals) have been shown to catalyze the ATP-dependent exchange of H2A.Z for H2A in the nucleosome. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. |
| 克隆类型： | Mouse Monoclonal Antibody |
| 亚型： | Mouse IgG2b |
| 免疫原： | Purified recombinant fragment of human TIP60 expressed in E. coli. |
| 适用物种： | H |
| 应用范围： | WB, IHC |
| 应用说明： | WB: 1:500-1:2000; IHC: 1:200-1:1000; |
| 别名： | TIP60 (HTATIP); Histone acetyltransferase; KAT5; 60 kDa Tat-interactive protein; Histone acetyltransferase HTATIP; HIV-1 Tat interactive protein; Lysine acetyltransferase 5; cPLA(2)-interacting protein; |
| 形态： | Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide. |
| 保存条件： | Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment. |
| 参考文献： |
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人热休克蛋白60 ( HSP60 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 HSP60 单抗包被于酶标板上，标准品和样品中的 HSP60与单抗结合，加入生物素化的抗人 HSP60 ，形成免疫复合物连接在板上，辣根
下，甚至短时间温度达到50-60℃，抗体分子都能维持正确折叠，保持应有的活性。 （3）抗体的纯度。采用先进的抗体纯化技术，确保抗体产品的高纯度，保障其抗体产品的稳定性。 （4）抗体的浓度。高浓度的抗体产品可减少容器表面吸附造成的物质损失，高浓度的抗体稳定性更好，抗体产品中93%的浓度大于0.5 mg/ml。 2. 抗体保存操作 抗体无论是保存还是运输都应尽可能地避免反复冻融，收到抗体后请根据实验的需要，对抗体进行分装，避免同一管抗体反复冻融（反复冻融会导致抗体
.Optional: Confirm successful protein transfer by Ponceau Red staining before proceeding to the next step.封闭，抗体孵育和洗膜1. Blocking: Incubate the blot in 3% non-fat milk / PBST or Trident Universal Protein Blocking Reagent for 30-60 minutes at RT.2. Primary
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