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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Bacterial fusion protein encompassing a 188-aa fragment from the C-term region of mouse periostin.
- 亚型:
Rabbit Ig
- 形态:
Each vial contains 0.1 ml IgG in PBS pH7.4 with 0.065% sodium azide. Antibody was purified by immunogen affinity chromatography.
- 保存条件:
Store at -20°C.
- 克隆性:
多克隆
- 标记物:
见下方详细说明
- 适应物种:
Ck, H, M, R
- 保质期:
1-2年
- 抗原来源:
Bacterial fusion protein encompassing a 188-aa fragment from the C-term region of mouse periostin.
- 目录编号:
253201
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Rabbit
- 应用范围:
E, WB, IHC
- 浓度:
见详细说明信息
- 靶点:
Periostin (C-term)
- 抗体英文名:
Periostin (C-term) Antibody
- 抗体名:
Periostin (C-term) Antibody
- 规格:
0.1 ml
Periostin (C-term) Antibody产品详细介绍:
| 产品名称: | Periostin (C-term) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 253201 |
| 规格: | 0.1 ml |
| 产品描述: | Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Periostin has since been demonstrated to be important in heart valve development and myocardial infarction because it promotes collagen fibrogenesis, inhibits differentiation of progenitor cells into cardiomyocytes and is essential in maintaining the biomechanical properties of the adult myocardium. This antibody detects a 93-kDa periostin doublet, which corresponds to the two largest forms of the alternatively spliced region of periostin with a distinctive staining pattern in IHC. |
| 克隆类型: | Rabbit Polyclonal Antibody |
| 亚型: | Rabbit Ig |
| 免疫原: | Bacterial fusion protein encompassing a 188-aa fragment from the C-term region of mouse periostin. |
| 适用物种: | Ck, H, M, R |
| 应用范围: | E, WB, IHC |
| 应用说明: | Cat. No. 253201 works well in IHC on paraformaldehyde-fixed sections with a citrate-based antigen retrieval protocol. E: 1:500-1:1,000; WB: 1:1,000; IHC: 1:100 |
| 别名: | Periostin; PN; Osteoblast-specific factor 2; OSF-2; Postn; Osf2 |
| 形态: | Each vial contains 0.1 ml IgG in PBS pH7.4 with 0.065% sodium azide. Antibody was purified by immunogen affinity chromatography. |
| 保存条件: | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献: |
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文献和实验于研究。 性能参数 LIST OF ANTBODY PRODUCTS AP8401a BDP1 N-term Antibody AP8408b MEG2 C-term Antibody AP8451b PPP3CA C-term Antibody AP8449b CTDSP1 C-term Antibody AP8408a MEG2 N-term Antibody AP8451a PPP3CA N-term Antibody AP8449
因子的C-term,做成融合蛋白。刺激细胞之后,对细胞进行化学交联,并破碎细胞以及基因组DNA,然后用anti-FLAG抗体作免疫沉淀,将转录因子沉淀下来。最后分析这些转录因子所结合的DNA片段序列,从而找到该转录因子的在基因组上的识别序列,也就找到了该转录因子的作用基因。 荧光素酶做报告基因模式小鼠,一般是为了做活体成像实验。比如在一个细胞因子启动子下面放置荧光素酶基因。免疫 小鼠之后,有些细胞或器官表达该细胞因子同时表达荧光素酶。通过注射荧光素酶底物可以检测荧光在小鼠体内的分布,从而得出该细胞
镐将HBx蛋白质注入正常肝细胞内,发现了它能激活肝细胞内的信息传导功能,而这种蛋白质能促进在细胞外起基质分解作用的MMP-9酶的生成,这种酶最终导致肝细胞发生癌变,诱发肝癌。 只要开发出能遏制由HBx蛋白质引发MMP-9酶生成的药物,便可以治疗和预防乙肝病毒引发的肝癌。 Duke大学医疗中心的研究人员一直在寻找与转移性的结肠癌有关的肿瘤相关基因,发现periostin在转移性的肿瘤中高度表现。当 periostin 引入实验室培养的人类结肠癌细胞时,细胞在移入小鼠体内后,更可能转移到肝脏中
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