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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of human HK2 expressed in E. coli.
- 亚型:
Mouse IgG1
- 形态:
Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide.
- 保存条件:
Store at -20°C
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 适应物种:
H
- 保质期:
1-2年
- 抗原来源:
Purified recombinant fragment of human HK2 expressed in E. coli.
- 目录编号:
253048
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
WB, IHC, IF
- 浓度:
见详细说明信息
- 靶点:
HK2 (3D3)
- 抗体英文名:
HK2 (3D3) Antibody
- 抗体名:
HK2 (3D3) Antibody
- 规格:
0.1 ml
HK2 (3D3) Antibody产品详细介绍:
| 产品名称: | HK2 (3D3) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 253048 |
| 规格: | 0.1 ml |
| 产品描述: | HK2 (Hexokinase-2) is an enzyme in the hexokinase family, playing a role in the carbohydrate metabolism. The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG1 |
| 免疫原: | Purified recombinant fragment of human HK2 expressed in E. coli. |
| 适用物种: | H |
| 应用范围: | WB, IHC, IF |
| 应用说明: | WB: 1:500-1:2000; IHC: 1:200-1:1000; IF: 1:200-1:1000; |
| 别名: | Hexokinase-2; Hexokinase type II; HK II; Muscle form hexokinase; HK2 |
| 形态: | Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide. |
| 保存条件: | Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
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从线粒体外膜脱落,进入细胞浆中。在细胞浆中,HK2 结合 NF-κB 的抑制因子 IκBα。 重要的是,HK2 发挥了不依赖于其经典代谢功能的新功能,即作为一个蛋白激酶磷酸化 IκBα 的 Thr 291 位点。该磷酸化促进了蛋白酶 μ-calpain 与 IκBα 的结合并进一步降解 IκBα,进而使转录因子 NF-κB 入核,最终促进了 PD-L1 的表达并导致了肿瘤的免疫逃逸。作者还发现,使用己糖激酶的抑制剂与 PD-1 抗体联用治疗小鼠胶质瘤,可以显著提升 PD-1 抗体的治疗
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