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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of PROZ expressed in E. coli.
- 亚型:
Mouse IgG1
- 形态:
Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide.
- 保存条件:
Store at -20°C
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 适应物种:
H
- 保质期:
1-2年
- 抗原来源:
Purified recombinant fragment of PROZ expressed in E. coli.
- 目录编号:
252902
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
WB
- 浓度:
见详细说明信息
- 靶点:
PROZ (2B4)
- 抗体英文名:
PROZ (2B4) Antibody
- 抗体名:
PROZ (2B4) Antibody
- 规格:
0.1 ml
PROZ (2B4) Antibody产品详细介绍:
| 产品名称: | PROZ (2B4) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 252902 |
| 规格: | 0.1 ml |
| 产品描述: | PROZ (Protein Z) is a 396 amino acids long (~62 kDa) vitamin K-dependent plasma glycoprotein. It has four domains: a gla-rich region, two EGF-like domains and a trypsin-like domain. It lacks the serine residue that would make it catalytically active as a serine protease. It is a member of the coagulation cascade, the group of blood proteins that leads to the formation of blood clots. It is vitamin K-dependent, and its functionality is therefore impaired in warfarin therapy. It is a glycoprotein. Although it is not enzymatically active, it is structurally related to several serine proteases of the coagulation cascade: factors VII, IX, X and protein C. The carboxyglutamate residues (which require vitamin K) bind protein Z to phospholipid surfaces. The main role of protein Z appears to be the degradation of factor Xa. This is done by protein Z-related protease inhibitor (ZPI), but the reaction is accelerated 1000-fold by the presence of protein Z. ZPI also degrades factor XI, but this reaction does not require the presence of protein Z. ZPI deficiency states have been associated with a propensity to thrombosis. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG1 |
| 免疫原: | Purified recombinant fragment of PROZ expressed in E. coli. |
| 适用物种: | H |
| 应用范围: | WB |
| 应用说明: | WB: 1:500-1:2000; |
| 别名: | Vitamin K-dependent protein Z; PROZ |
| 形态: | Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide. |
| 保存条件: | Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
ABBIOTEC产品提供质量保证,提供完善的售后和技术支持,ABBIOTEC产品正品包装:
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文献和实验Stewart Laboratory Standard Operating Procedure
+ 0.5% IFS (make fresh!). Incubate at RT for 20 minutes. Wash cells once with 1 mL wash buffer. Pellet and resuspend in 0.1 M sodium tetraborate (Na2B4O7). Incubate at RT for 2 minutes. Wash cells twice with 1 mL wash buffer (if a negative control of PI
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-20℃保存。 为最大限度保持抗体活性,整个过程应在4℃下进行。对于冻干抗血清或长时间保存的血清,将生理盐水透析改为3 M KCNS透析,促使聚合的多聚体解聚。如果抗血清效价较低时,不宜准备胶体金探针。 2、亲和纯化抗体的处理 亲和纯化抗体多是一些商品化的通用抗体,即二抗。一般为羊抗兔、羊抗鼠或羊抗人的抗体。这些抗体一般有两个问题,一是IgG分子往往聚集为多聚体分子,二是往往含有较多的盐分。因此前处理的目的在于脱盐和解聚。 其基本步骤如下: (1) 将亲和纯化抗体用生理
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