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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
江苏省授权一级代理商
- 库存:
大量
- 供应商:
无锡莱弗思生物实验器材有限公司
- 规格:
25T
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文献和实验E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
again. Centrifuge at maxi speed (13,000 x g) for 3 minutes at room temperature (22-25℃). The ETR Reagent should form a blue layer at bottom of the tube. 7. Transfer the top aqueous phase (cleared lysate) into a new 2 ml microtube and add 0.5 volume of absolute
Fastfilter Plasmid Maxi Kit Spin Protocol
. All of centrifugation steps must be carried out at room temperature. Purify Plasmid DNA with HiBindTM DNA Maxi Column: 10. Transfer 16 ml of the cleared lysate to the HiBind® DNA maxi column assembled in the 50 ml collection tube. The Maxi column
E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
实验步骤 1. Inoculate 5ml LB/ampicillin (50 ug/ml) medium placed in a 10-20 ml culture tube with E.coli carrying desired plasmid and grow at 37°C with agitation for 12-16 h. It is strongly recommended that an endA negative
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