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- 2025年12月05日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C, sealed storage, away from moisture and light
- 库存：
货期：1-2天
- 供应商：
MedChemExpress LLC
- CAS号：
64987-85-5
- 规格：
100 mg/250 mg/500 mg/1 g/5 g/25 g
| 规格： | 100 mg | 产品价格： | ￥350.0 |
|---|---|---|---|
| 规格： | 250 mg | 产品价格： | ￥500.0 |
| 规格： | 500 mg | 产品价格： | ￥675.0 |
| 规格： | 1 g | 产品价格： | ￥1012.0 |
| 规格： | 5 g | 产品价格： | ￥1820.0 |
| 规格： | 25 g | 产品价格： | ￥3370.0 |
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SMCC
CAS No. : 64987-85-5
MCE 国际站：SMCC
产品活性：SMCC 是一种蛋白质交联剂。SMCC 接合抗原耦合脾脏细胞来诱导抗原特异性免疫反应。
研究领域：Antibody-drug Conjugate/ADC Related
作用靶点：ADC Linker
In Vitro: SMCC or sulfo-SMCC can use to conjugate keyhole limpet hemocyanin (KLH) and ovalbumin (OVA). In the the conjugation system, 300 μl KLH or OVA (3 mg) and 30 μl SMCC or Sulfo-SMCC (the final concentration of 0.1 mM) are added in PBS with final volume of 3 ml.
In Vivo: Using the SMCC-conjugation method is able to induce potent immune responses to whole protein or peptide antigens by injection of antigen-coupled syngeneic splenic mononuclear cells in mice.
相关产品：DSS Crosslinker | Mc-Val-Cit-PABC-PNP | DBCO-NHS ester | Sulfo-SMCC sodium | MC-Val-Cit-PAB | DSG Crosslinker | DSP Crosslinker | Val-cit-PAB-OH | Fmoc-3VVD-OH | SPDP | Fmoc-Val-Cit-PAB | MC-Gly-Gly-Phe-Gly-NH-CH2-O-CH2COOH | Fmoc-8-amino-3,6-dioxaoctanoic acid | Fmoc-Val-Cit-PAB-PNP | MAC glucuronide linker-2 | Maleimide-DOTA | N3-PEG4-C2-NHS ester | BS3 Crosslinker disodium | Fmoc-Gly-Gly-Phe-OH | N-Hydroxysulfosuccinimide sodium | Boc-Dap-NE | DBCO-acid | Desthiobiotin-Iodoacetamide | Vipivotide tetraxetan Ligand-Linker Conjugate | DBCO-amine | N-Butanoyl-L-homoserine lactone | Fmoc-NH-PEG4-CH2CH2COOH | SPDB | DOTA-NHS-ester | DBCO-PEG5-NHS ester
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文献和实验Affinity Purification Of Antibodies
in NNdimethylformamide.(3) Mix 20ml SMCC and 200ml BSA (500mg:4000mg or 1:8); incubate ON RT.(4) Separate conjugate from unreacted crosslinker on a G25 column in 100mM phospate, pH 6.2. Identify the peak by A280 and pool peak (1 ml) fractions.(5) Dissolve peptide (1-5 mg
AFFINITY PURIFICATION OF ANTIBODIES
Conjugation of carrier (BSA) to peptide 1. Dissolve pure BSA @ 20mg/ml in 10mM phosphate, pH 7.0. 2. Dissolve MBS-SMCC [m-maleimidobenzoyl N-hydroxysuccinimide ester succinimidyl 4- (N-maleimidomethyl)cyclohexane-1-carboxylate] @ 25
AFFINITY PURIFICATION OF ANTIBODIES
AFFINITY PURIFICATION OF ANTIBODIES (Fisher et al.,1988, Cell 54: 813-822) Conjugation of carrier (BSA) to peptide 1. Dissolve pure BSA @ 20mg/ml in 10mM phosphate, pH 7.0. 2. Dissolve MBS-SMCC [m-maleimidobenzoyl N-hydroxysuccinimide
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