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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海机纯实业有限公司
- 库存:
95
- 规格:
5×105 cells/细胞冻存管
产品规格:5×105 cells/细胞冻存管
保存条件:干冰运输,液氮保存。不建议长期保存。
组织来源:正常SD大鼠。
SD
90%
推荐培养液成分
DMEM/F12;2.5% 胎牛血清;EGF:2nmol/L;胰岛素:2.5mg/mL。
使用方法
收到细胞后,请将细胞放入液氮中冻存或直接按以下步骤复苏细胞:
1.从液氮或干冰中取出冻存管迅速放入37℃水浴中,并不时摇动,在1分钟内使其完全融化。
2.吸出细胞,800 g/min离心5分钟,弃上清,加入适量培养液重悬细胞后接种于培养瓶中,接种浓度5×105/ml,置37℃,5% CO2细胞培养箱中培养;或无需离心直接将细胞加入培养液贴壁培养12-24小时后,换新鲜培养液继续培养。待细胞状态稳定后用于后续实验。
注意事项
Rat Corneal Epithelial Cells大鼠肺泡上皮细胞该细胞只可用于科研,不推荐长期冻存或传代培养,请在收到细胞后尽快使用。
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