- 询价
- Biotechrabbit
- Biotechrabbit
- BR0100102
- 2025年08月06日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Taq DNA Polymerase (wild-type), 5 U/µl
- 供应商：
默瑞（上海）生物科技有限公司
- 规格：
500 U
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文献和实验product is obtained. 5.Taq DNA Polymerase. Usually 1-1.5u of Taq DNA Polymerase are used in 50µl of reaction mix. Higher Taq DNA Polymerase concentrations may cause synthesis of nonspecific products. However, if inhibitors are present in the reaction
Single tube confirmation PCR protocol
Polymerase. - Add 35 µl of the PCR mix to each of the 20 PCR tubes that already contain the 15 µl of primers and template. 110 µl 5 µl 10 x Taq buffer(see below) 11 µl 0.5 µl 20 mM dNTP's (0.2 mM) 11 µl 0.5 µ
x PCR reaction buffer, 1.6 µl 4dNTP mix (250 pmol/µl), 2 µl T12 MN primer, 2 µl arbitrary primer (decamer), 0.2 µl Taq DNA polymerase (5 U/µl) use the PCR conditions as under section III. run PCR product on a 1.5 % agarose gel
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