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- 询价
- Signalway Antibody
- 27578
- 2025年09月23日
- WB,IHC,IF
- Rabbit
- Human;Mouse;Rat
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant fusion protein of human Vimentin (NP_003371.2).
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Polyclonal
- 适应物种：
Human;Mouse;Rat
- 保质期：
12 months
- 抗原来源：
Rabbit
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB,IHC,IF
- 靶点：
Uniprot：P08670
- 抗体英文名：
Vimentin Polyclonal Antibody
- 规格：
50ul/100ul
计算分子量:57kDa
配方:Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.
应用详情:
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
图片:
Western blot analysis of extracts of various cell lines, using Vimentin at 1:1000 dilution.
,
Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin at 1:1000 dilution.
,
Immunohistochemistry of paraffin-embedded human tonsil using Vimentin at dilution of 1:150 (40x lens).
,
Immunohistochemistry of paraffin-embedded human stomach using Vimentin at dilution of 1:150 (40x lens).
,
Immunofluorescence analysis of L929 cells using [KO] Vimentin at dilution of 1:100. Blue: DAPI for nuclear staining.
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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