- ￥1600 - 2650
- MultiSciences
- 31.25 pg/mL - 2000 pg/mL
- 杭州
- EK210
- 2026年04月13日
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- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
联科生物
- 检测范围：
31.25 pg/mL - 2000 pg/mL
- 检测方法：
双抗夹心法
- 应用：
ELISA
- 适应物种：
Mouse
- 标记物：
HRP
- 样本：
血清，血浆，细胞培养上清及其他生物学样本
- 规格：
96T/48T
| 规格： | 96T | 产品价格： | ￥2650.0 |
|---|---|---|---|
| 规格： | 48T | 产品价格： | ￥1600.0 |
| 背景介绍 | 白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中，它由位于1号染色体的IL-10基因编码，由5个外显子组成。IL10主要由单核细胞分泌，淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性，并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床，抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。 |
| 检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-10与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-10的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。 |
| 组分 | 包被抗IL-10单克隆抗体的96孔聚苯乙烯酶标板 小鼠IL-10冻干标准品 IL-10检测抗体 标准品稀释液 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) 封板膜 |
| 分析类型 | 夹心ELISA |
| 样本类型 | 血清、血浆，细胞培养上清及其他生物学样本 |
| 板式 | 96孔板，可拆 |
| 保存 | 试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 样本体积 | 10 μl |
| 灵敏度 | 4.8 pg/ml |
| 标准曲线范围 | 125 - 8000 pg/ml |
| 回收率范围 | 86 %-108 % |
| 平均回收率 | 0.97 |
| 板间变异系数 | 5.3 % - 7.1 % |
| 板内变异系数 | 3.8 % - 8.8 % |
小鼠白介素10 ELISA试剂盒说明书
本产品文献引用3篇，详细如下：
【1】Ma Lingman, Wang Yanrong, Wang Mengxiao, et al. Effective antimicrobial activity of Cbf-14, derived from a cathelin-like domain, against penicillin-resistant bacteria[J]. Biomaterials, 2016, 87: 32-45 (IF:8.557) |
【2】Xiangliang D, Shuang L, Xia L, et al. Polysaccharides from Chinese Herbal\r, Lycium barbarum\r, Induced Systemic and Local Immune Responses in H22 Tumor-Bearing Mice[J]. Journal of Immunology Research, 2018, 2018: 1-12 (IF:3.298) |
【3】Wang Y, Gu Y, Fang K, et al. Lactobacillus acidophilus\r, and\r, Clostridium butyricum\r, ameliorate colitis in murine by strengthening the gut barrier function and decreasing inflammatory factors[J]. Beneficial Microbes, 2018: 1-14 (IF:2.3) |
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相关实验
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠白介素-10 (IL-10)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 IL-10 单抗包被于酶标板上，标准品和样品中的 IL-10与单抗结合，加入生物素化的抗小鼠
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人白介素-10 (IL-10)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 IL-10 单抗包被于酶标板上，标准品和样品中的 IL-10与单抗结合，加入生物素化的抗人 IL-
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 兔白介素-10 (IL-10)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗兔 IL-10 单抗包被于酶标板上，标准品和样品中的 IL-10与单抗结合，加入生物素化的抗兔 IL-10
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Mouse IL-10 ELISA Kit（小鼠白介素10 ELISA试剂盒）
￥1600 - 2650
