- ￥1600 - 2650
- MultiSciences
- 312.5 pg/mL - 20000 pg/mL
- 杭州
- EK268
- 2026年03月30日
企业认证
研选同类产品更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 用户评价
- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
联科生物
- 检测范围：
312.5 pg/mL - 20000 pg/mL
- 检测方法：
双抗夹心法
- 应用：
ELISA
- 适应物种：
Mouse
- 标记物：
HRP
- 样本：
血清，血浆，细胞培养上清及其他生物学样本
- 规格：
96T/48T
| 规格： | 96T | 产品价格： | ￥2650.0 |
|---|---|---|---|
| 规格： | 48T | 产品价格： | ￥1600.0 |
| 背景介绍 | C-X-C基序趋化因子10(CXCL10)，又名γ干扰素诱导蛋白10(IP-10)，是一个分子量为8.7kDa的蛋白，属于CXC趋化因子家族。多种细胞类型包括单核细胞、内皮细胞和成纤维细胞在响应IFN-γ时可分泌IP-10。IP-10具有多种作用功能，如趋化单核/巨噬细胞、NK细胞和树突状细胞，促进T细胞粘附到内皮细胞，抗肿瘤活性，抑制骨髓集落形成及抑制血管生成。
IP-10与趋化因子MIG拥有共同受体CXCR3，但是在感染的宿主防御过程中发挥不同的作用。IP-10参与过敏性接触性皮炎反应和其它过敏性疾病，在免疫介导的过程、神经系统炎症和阿尔茨海默病中出现。也有研究报道它可参与调节炎症性肺损伤后的纤维增生。 |
| 检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IP-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IP-10与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IP-10的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570-630 nm)测定吸光度值。 |
| 组分 | 包被抗CXCL10/IP-10单克隆抗体的96孔聚苯乙烯酶标板 小鼠CXCL10/IP-10冻干标准品 CXCL10/IP-10 检测抗体 标准品稀释液 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) 封板膜 |
| 分析类型 | 夹心ELISA |
| 样本类型 | 血清、血浆，细胞培养上清及其他生物学样本 |
| 板式 | 96孔板，可拆 |
| 保存 | 试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 样本体积 | 血清血浆：50 μl 细胞培养上清：100μl |
| 灵敏度 | 19.03 pg/ml |
| 标曲图 |  |
| 标准曲线范围 | 312.5-20000pg/ml |
| 回收率范围 | 71%-122% |
| 平均回收率 | 0.95 |
| 板间变异系数 | 1.3%-3.8% |
| 板内变异系数 | 2.2%-5.3% |
CXCL10 分子靶点信息概述
- 分子名：CXCL10, C-X-C motif chemokine ligand 10
- 基因家族：Chemokine ligands
- 别名：IFI10; IP-10; crg-2; mob-1; C7; gIP-10
- 曾用名：INP10; SCYB10
- 全称：small inducible cytokine subfamily B (Cys-X-Cys), member 10; chemokine (C-X-C motif) ligand 10
CXCL10 分子靶点综述
趋化因子配体10(CXCL10)又称为γ-干扰素诱导蛋白10(IP-10)或微小诱导细胞因子B10，是属于CXC趋化因子家族的小分子量细胞因子。多种类型的细胞对IFN-γ产生应答后可分泌IP-10，包括单核细胞、内皮细胞和成纤维细胞等。IP-10具有多种功能，如对单核/巨噬细胞、T细胞、NK细胞和树突细胞的趋化作用，促进T细胞黏附于内皮细胞，抗肿瘤活性和抑制骨髓集落形成和血管生成。
小鼠 Mouse Cxcl10 分子靶点信息
- 分子名：Cxcl10, chemokine (C-X-C motif) ligand 10
- 别称：
- C7
- CRG-2
- gIP-10
- Ifi10
- INP10
- interferon activated gene 10
- IP-10
- IP10
- mob-1
- Scyb10
- small inducible cytokine B subfamily (Cys-X-Cys), member 10
- 基因序列：NCBI_Gene: 15945
- 蛋白序列：
- UniprotKB: P17515
- UniprotKB: D3YW23
- UniprotKB: Q3U6X5
- UniprotKB: Q3UAS6
- UniprotKB: Q3UCV1
- UniprotKB: Q3UK71
- UniprotKB: Q548V9
小鼠 Mouse Cxcl10 靶点分子功能（预测）
Predicted to enable chemoattractant activity; chemokine receptor binding activity; and heparin binding activity. Acts upstream of or within several processes, including defense response to virus; negative regulation of myoblast differentiation; and negative regulation of myoblast fusion. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including alimentary system; axial skeleton; hemolymphoid system; and liver. Human ortholog(s) of this gene implicated in hepatitis B; middle cerebral artery infarction; and type 1 diabetes mellitus. Orthologous to human CXCL10 (C-X-C motif chemokine ligand 10).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
用户评价
暂无用户评价
文献和实验- IF = 20.722 Fullerenols boosting the therapeutic effect of anti-CD47 antibody to trigger robust anti-tumor immunity by inducing calreticulin exposure. Nano Today (2021)
- IF = 14.978 Hypoxia-Inducible Factor 2α Attenuates Renal Ischemia-Reperfusion Injury by Suppressing CD36-Mediated Lipid Accumulation in Dendritic Cells in a Mouse Model. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY (2023)
- IF = 14.957 Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases. Science Advances (2022)
- IF = 11.614 Cytosolic delivery of the immunological adjuvant Poly I:C and cytotoxic drug crystals via a carrier-free strategy significantly amplifies immune response. Acta Pharmaceutica Sinica B (2021)
- IF = 10.245 Lacticaseibacillus paracasei sh2020 induced antitumor immunity and synergized with anti-programmed cell death 1 to reduce tumor burden in mice. Gut Microbes (2022)
- IF = 9.756 FOSL2 promotes intertumoral infiltration of T cells and increases pathological complete response rates in locally advanced rectal cancer patients. CANCER LETTERS (2023)
- IF = 8.146 A non-bactericidal cathelicidin provides prophylactic efficacy against bacterial infection by driving phagocyte influx. eLife (2022)
- IF = 7.723 CXCL10-armed oncolytic adenovirus promotes tumor-infiltrating T-cell chemotaxis to enhance anti-PD-1 therapy. OncoImmunology (2022)
- IF = 7.419 Peucedanum praeruptorum Dunn polysaccharides regulate macrophage inflammatory response through TLR2/TLR4-mediated MAPK and NF-κB pathways. BIOMEDICINE & PHARMACOTHERAPY (2022)
- IF = 6.986 Viperin deficiency promotes dendritic cell activation and function via NF-kappaB activation during Mycobacterium tuberculosis infection. INFLAMMATION RESEARCH (2022)
- IF = 6.953 Molecular mechanisms underlying macrophage immunomodulatory activity of Rubus chingii Hu polysaccharides. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)
- IF = 6.244 CDKN2A Deletion in Melanoma Excludes T Cell Infiltration by Repressing Chemokine Expression in a Cell Cycle-Dependent Manner. Frontiers in Oncology (2021)
- IF = 5.811 MiR-19a-3p Suppresses M1 Macrophage Polarization by Inhibiting STAT1/IRF1 Pathway. Frontiers in Pharmacology (2021)
- IF = 5.811 Isoforskolin Alleviates AECOPD by Improving Pulmonary Function and Attenuating Inflammation Which Involves Downregulation of Th17/IL-17A and NF-κB/NLRP3. Frontiers in Pharmacology (2021)
- IF = 5.546 miR‐130b‐3p regulates M1 macrophage polarization via targeting IRF1. JOURNAL OF CELLULAR PHYSIOLOGY (2020)
- IF = 5.293 CX3CL1 Recruits NK Cells Into the Central Nervous System and Aggravates Brain Injury of Mice Caused by Angiostrongylus cantonensis Infection. Frontiers in Cellular and Infection Microbiology (2021)
- IF = 4.886 Combination of Fruquintinib and Anti–PD-1 for the Treatment of Colorectal Cancer. JOURNAL OF IMMUNOLOGY (2020)
- IF = 4.344 Phospho-Tyr705 of STAT3 is a therapeutic target for sepsis through regulating inflammation and coagulation. Cell Communication and Signaling (2020)
- IF = 3.684 Mast cell degranulation impairs pneumococcus clearance in mice via IL-6 dependent and TNF-α independent mechanisms. World Allergy Organization Journal (2019)
- IF = 2.939 Inhibition of Fibroblast Growth Factor Receptor by AZD4547 Protects Against Inflammation in Septic Mice. INFLAMMATION (2019)
- IF = 2.838 Qin Huang formula enhances the effect of Adriamycin in B-cell lymphoma via increasing tumor infiltrating lymphocytes by targeting toll-like receptor signaling pathway. BMC Complementary Medicine and Therapies (2022)
- IF = 2.835 Oral administration of MnCl2 attenuated hyperlipidemia-related cardiac remodeling in ApoE−/− mice. JOURNAL OF PHARMACOLOGICAL SCIENCES (2020)
- IF = 2.65 Prevention of Bleomycin-Induced Pulmonary Inflammation and Fibrosis in Mice by Bilobalide. Evidence-based Complementary and Alternative Medicine (2023)
- IF = 1.798 Oroxylin A inhibited autoimmune hepatitis-induced liver injury and shifted Treg/Th17 balance to Treg differentiation. EXPERIMENTAL ANIMALS (2023)
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人IP-10 (I P - 10 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液、唾液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 IP-1 0 /CXCL10 ( interferon-inducible protein
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内，每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激，我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤，分别固定，打孔，猝灭，封闭，然后一抗孵育，二抗孵育，最后显色，用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正，最后得出以上数据。Quantitative
技术资料需要更多技术资料 索取更多技术资料
