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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测鸡血清,血浆等样本中目标蛋白的浓度
- 适应物种:
鸡
- 标记物:
Chicken N(6)-Carboxymethyllysine,CML
- 样本:
鸡的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Chicken Carboxymethyl lysine, CML ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
N(6)-Carboxymethyllysine,; N(epsilon)-(carboxymethyl)lysine, CML , Carboxymethyl lysine
Search name
Chicken Carboxymethyl lysine ELISA Kit, Chicken CML ELISA Kit, Chicken N(6)-Carboxymethyllysine ELISA Kit, Chicken
N(epsilon)-(carboxymethyl)lysine ELISA Kit, Carboxymethyl lysine ELISA Kit, CML ELISA Kit, N(6)-Carboxymethyllysine ELISA
Kit, N(epsilon)-(carboxymethyl)lysine ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of
N(6)-Carboxymethyllysine concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to N(6)-Carboxymethyllysine, During the reaction, N(6)-Carboxymethyllysine in the sample or
standard competes with a fixed amount of biotin-labeled N(6)-Carboxymethyllysine for sites on a pre-coated Monoclonal antibody specific
to N(6)-Carboxymethyllysine. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of N(6)-Carboxymethyllysine in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
"(本产品仅供体外科研用途,不可用于临床诊断!)
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文献和实验(positive),而是病人(patient)的缩写,不应误解。为避免混淆,更宜用 S/N 表示。在早期的间接法 ELISA 中,有些作者定出 S/N 为阳性标准,现多为各种测定所沿用。实际上每一测定系统应该用实验求出各自的 S/N 的阈值。更应注意的是,N 所代表的阴性对照是不含受检物质的人血清。有的试剂盒中所设阴性对照为不含蛋白质或蛋白质含量较底的缓冲液,以致反应后产生的本底可能较正常人血清的本底低得多。因此,这类试剂盒规,如 N (2) 竞争法 在竞争法 ELISA 中,阴性孔呈色深于阳性孔。阴性
质控时使用。 下面再解释一下在ELISA定性测定结果判定中常用的一些缩写。 (1)S/CO:其中S为Sample(样本)或Specimen(标本)的简写,表示的是标本测定的吸光度值,CO为Cut-off值的简写。除竞争抑制法外,其它ELISA定性测定模式中,当S/CO值大于或等于1时,标本的测定为阳性,小于1时为阴性。 (2)S/N或P/N:其中S同(1),N为Negative(阴性对照)的简写,P为Patient(患者)的简写。较早的试剂盒很多都使用S/N或 P/N≥2.1为阳性
抗体的结构 抗体是能与抗原特异性结合的免疫球蛋白(immunoglobulin,Ig)。Ig 分五类,即 IgG、IgA、IgM、IgD 和 IgE。与免疫测定有关的 Ig 主要为 IgG 和 IgM。Ig 由两个轻链(L)和两个重链(H)的单体组成。Ig 的轻链是相同的,有 κ(kappa)和 λ(Lambda)两种型别。五类 Ig 的重链结构不同,这决定了它们的抗原性也不同。IgG 和 IgM 的重链分别称为 γ(gamma)链和 μ(mu )链。 重链和轻链的 N 端的氨基酸排列顺序
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