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- 英文名:
1×TBS(Powder)Tri
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上海经科化学科技有限公司
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文献和实验on APES coated slides, they are best kept at -80°C until needed. When required, leave to warm at room temperature for 5 minutes, then acetone fix for 5 minutes followed by a PBS or TBS rinse. Afterwards, continue with the immunohistochemical staining
DIRECT ELISA USING FLUORESCENT SUBSTRATE PROTOCOL
Day 1:1. Coat with 100 μl/well of coating antibody diluted in filtered PBS. Incubate plate overnight at 4°C, coveredwith plate sealer.Day 2:2. Block plates with 200 μl/well of 4 g Block ACE powder, diluted in 100 ml of deionized water (USE a 1
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
, UK). 3. Red Ponceau solution (1×): 0.2% red Ponceau powder, 3% trichloroaceticacid (TCA) in water. Store at room temperature. 2.6. Agarose Gel Electrophoresis to Detect Full Length APC (~300 kDa) 1. Laemmli sample buffer (4×): 250 mM
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