- ￥2260
- LSM Bio
- 进口
- PAab10007
- 2025年07月17日
- ELISA, WB, IHC
- E. coli - derived recombinant protein
- Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
protein kinase C, gamma
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
polyclonal
- 标记物：
Non-conjugated
- 适应物种：
Mouse, Rat
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
Q9BZJ3
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA, WB, IHC
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
protein kinase C, gamma
- 抗体英文名：
PKC gamma antibody
- 抗体名：
PKC gamma antibody
- 规格：
100μl
PKC gamma antibody
Product Name PKC gamma antibody
Catalog No PAab10007
Packing 100ul
Form liquid
Alternative Name PKC gamma, PKCC, PKCG, PRKCG, Protein kinase C gamma type, protein kinase C, gamma, SCA14
Purification Immunogen affinity purified
Purity 95% as determined by SDS-PAGE
Host Rabbit
Isotype IgG
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
Protein kinase C(PKC) is a family of serine-and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14(SCA14), an autosomal dominant neurodegenerative disease.
Immunogen protein kinase C, gamma
Specificity Mouse, Rat
Tested Application ELISA, WB, IHC
Recommended dilution WB: 1:500-1:2000; IHC: 1:100-1:500
IHC use Immunohistochemistry of paraffin-embedded rat brain tissue slide using PAab10007(PRKCG Antibody) at dilution of 1:200
WB use
mouse brain tissue were subjected to SDS PAGE followed by western blot with PAab10007(PRKCG antibody) at dilution of 1:1000
Protein Information
Gene ID
Uniprot ID P63318
Calculated MW 78 kDa
Research Area Signal Transduction, Metabolism, Cardiovascular, Immunology, Neuroscience
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文献和实验提示我模型中的通路究竟涉及哪个亚型，而我又没有那么多经费一个一个亚型去试，故我想能不能就检测总的PKC激活水平，毕竟有亚型被激活，那总的活化PKC水平也应该会升高啊！虽然敏感性和特异性肯定没有单独检测一个亚型的高。 还想请教：Phospho-PKC (pan) (gamma Thr514) Antibody 和Phospho-PKC (pan) (βII Ser660) Antibody 这两个抗体的针对的亚型有什么不同？既然都是pan，那是否说都是针对所有的亚型，只是磷酸化的残基不同
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠蛋白激酶 C(PKC)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 PKC 单抗包被于酶标板上，标准品和样品中的 PKC 与单抗结合，加入生物素化的抗大鼠 PKC ，形成免疫复合物连接在板上，辣根
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人蛋白激酶C (PKC)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 PKC 单抗包被于酶标板上，标准品和样品中的 PKC与单抗结合，加入生物素化的抗人 PKC ，形成免疫复合物连接
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