PARP1 antibody
Product Name PARP1 antibody
Catalog No PAab09939
Packing 100ul
Form liquid
Alternative Name ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL
Purification protein A+G purified
Purity 95% as determined by SDS-PAGE
Host Mouse
Isotype IgG1
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis.
Immunogen poly (ADP-ribose) polymerase 1
Specificity Human, Mouse, Rat
Tested Application ELISA, WB, IHC, IF
Recommended dilution WB: 1:5000 - 1:30000; IHC: 1:100 - 1:1000; IF: 1:50 - 1:500
IHC use Immunohistochemistry of paraffin-embedded human breast cancer using PAab09939(PARP1 antibody) at dilution of 1:600
WB use
HeLa cells were subjected to SDS PAGE followed by western blot with PAab09939(PARP1 Antibody) at dilution of 1:20000
Protein Information
Gene ID 142
Uniprot ID P09874
Calculated MW 115 kDa, 89 kDa
Research Area Metabolism