- ￥2260
- LSM Bio
- 进口
- PAab09847
- 2025年07月17日
- ELISA, WB, IHC, IF
- E. coli - derived recombinant protein
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
nicotinamide phosphoribosyltransferase
- 亚型：
IgG2b
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
monoclonal
- 标记物：
Non-conjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
6个月
- 抗原来源：
Mouse
- 目录编号：
Q9BZJ3
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA, WB, IHC, IF
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
nicotinamide phosphoribosyltransferase
- 抗体英文名：
Visfatin antibody
- 抗体名：
Visfatin antibody
- 规格：
100μl
Visfatin antibody
Product Name Visfatin antibody
Catalog No PAab09847
Packing 100ul
Form liquid
Alternative Name 1110035O14Rik, DKFZp666B131, NAmPRTase, NAMPT, PBEF, PBEF1, Pre B cell enhancing factor, VF, VISFATIN
Purification protein A+G purified
Purity 95% as determined by SDS-PAGE
Host Mouse
Isotype IgG2b
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus(T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.
Immunogen nicotinamide phosphoribosyltransferase
Specificity Human, Mouse, Rat
Tested Application ELISA, WB, IHC, IF
Recommended dilution WB: 1:1000-1:4000; IHC: 1:50-1:500; IF: 1:50-1:500
IHC use Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using PAab09847( Visfatin antibody ) at dilution of 1:200
WB use
rat heart tissue were subjected to SDS PAGE followed by western blot with PAab09847( Visfatin antibody ) at dilution of 1:2000
Protein Information
Gene ID 10135
Uniprot ID P43490
Calculated MW
Research Area Neuroscience, Immunology, Signal Transduction, Metabolism, Cardiovascular
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠内脂素 (Visfatin)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 Visfatin 单抗包被于酶标板上，标准品和样品中的 Visfatin 与单抗结合，加入生物素化的抗大鼠 Visfatin
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 Visfatin 定量 EIA 试剂盒使用说明 原理 本实验采用双抗体夹心 ELISA 法。用抗人 Visfatin 单抗包被于酶标板上，标准品和样品中的 Visfatin 与单抗结合，加入酶标抗体，形成免疫复合物连接在板上，加入酶底物TMB ，出现蓝色，加终止液硫酸，颜色变黄
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
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