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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
2年
- 英文名:
Amphotericin B solubilized
- 库存:
大量
- 供应商:
上海瓦兰生物
- CAS号:
1397-89-3
- 保存条件:
2-8℃
- 规格:
1g/5g
| 规格: | 1g | 产品价格: | ¥115.0 |
|---|---|---|---|
| 规格: | 5g | 产品价格: | ¥337.0 |
化合物简介
基本信息
中文别名:两性霉素 B;芦山霉素;二性霉素B;
英文别名:LNS-AmB;AMBISOME;Amphocin;fungilin;Amphotericin B;Halizon;ns718;FUNGIZONE;AMPHOZONE;Abelecet;ABELCET;
分子式:C47H73NO17
分子量:924.079
精确质量:923.488
Psa:319.61
Logp:1.412
编号系统
MDL号:MFCD00877763
RTECS号:BU2625000
物化性质
密度:1.34
沸点:1140.4ºC at 760 mmHg
熔点:>170°C
闪点:643.5ºC
折射率:1.614
稳定性:Stable, but may be light sensitive. Incompatible with strong oxidizing agents.
储存条件:库房通风低温干燥
蒸汽压:0mmHg at 25°C
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文献和实验at least 2 mg (up to 4mg) of total RNA in 750 l RNase-free water. In general, inputting more total RNA will yield more polyA RNA. This protocol is most successful with inputs of at least 2 mgs. (Less than 1mg of total RNA input is NOT recommended
Procedure for Transfection of Mammalian Cells
in a CO2 incubator until the cells are 70-80% confluent. This will usually take 18-24 h. Prepare the following solutions in 12 x 75 mm sterile tubes: Solution A: For each transfection, dilute 2 μg DNA (plasmid) in 375 μl serum-free IMDM
Single tube confirmation PCR protocol
(200mg/l). - Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants. 2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution
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