Rat CCK- 8 ELISA Kit allows for the in vitro quantitative determination of CCK-8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
适应物种:
大鼠
标记物:
详见说明书
样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
规格:
96T
Rat CCK- 8 ELISA Kit
Product Name:Rat CCK- 8 ELISA Kit Packing:96T
Catalog No.:ELA-E1044r
Gene Name: CCK-8
Detect Range:31.2-2000 pg/mL
Sensitivity:10pg/mL
Target Protein Name: CCK-8
Alternative Name: CCK-8 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Rat CCK- 8 ELISA Kit allows for the in vitro quantitative determination of CCK-8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human LRP2 ELISA Kit has been pre-coated with an LRP2 antibody specific to Human Low-density lipoprotein receptor-related protein 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Low-density lipoprotein receptor-related protein 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Low-density lipoprotein receptor-related protein 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Low-density lipoprotein receptor-related protein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!