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- 英文名:
Corning® Collagen IV, Mouse, 1mg
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文献和实验Primary Mouse Keratinocyte Culture
we describe will be especially useful for the isolation and analysis of cells harvested from transgenic or knockout mice. We explain how to use a supplemented fibroblast-conditioned medium, along with mouse collagen IV-coated culture dishes, to establish and subculture
Generation of Functional Multipotent Keratinocytes from Mouse Induced Pluripotent Stem Cells
and efficiency of keratinocyte derivation depending on the mouse genetic background used in the study. Both protocols employ applications of retinoic acid and bone-morphogenetic protein-4 and growth on collagen type IV-coated dishes to induce iPSC differentiation
they may be split 1:4 into fresh media. (Fungizone can be omitted from this point onwards). 2. Stage I - Preparation of collagen I from rat tails Note: Protocol for approximately 12-14 adolescent (fresh or frozen) rat tails 1) Prepare rat
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