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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Rat EGFR / HER1 / ErbB1 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
50.00 µg/100.00 µg
| 规格: | 50.00 µg | 产品价格: | ¥2310.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥3870.0 |
蛋白名称:EGFR蛋白, EGFR protein
蛋白构建:A DNA sequence encoding the rat EGFR (E7CXR8) (Met1-Ser646) was expressed, fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 98 % as determined by SDS-PAGE
蛋白活性:1. Measured by its ability to bind recombinant human EGF-Fc (Cat:10605-H01H) in a functional ELISA. 2. Measured by its ability to bind recombinant mouse EGF-Fc (Cat:50482-M01H) in a functional ELISA.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Leu 25
蛋白分子量:The recombinant rat EGFR comprises 633 amino acids and predicts a molecular mass of 70.7 kDa. The apparent molecular mass of the recombinant protein is approximately 93-110 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:E7CXR8
蛋白氨基酸序列:Met1-Ser646
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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- 作者
- 内容
- 询问日期
文献和实验Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
in recombinant Escherichia coli, Biochem. J. 358 (2001) 263-268. 35. G. Lemercier, N. Bakalara, X. Santarelli, On-column refolding of an insoluble histidine tag recombinant exopolyphosphatase from Trypanosoma brucei overexpressed in Escherichia coli, J
ASTRACT We describe a rapid and quantitative flow cytometric method for determining the apoptotic or anti-apoptotic potential of a gene in various cell types. A plasmid carrying green fluorescent protein (GFP) is co-transfected
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