Recombinant Human FCGRT&B2M Heterodimer Protein(His&AVI Tag),Biotinylated
库存:
99
供应商:
北京义翘神州科技股份有限公司
规格:
20 µg/100 µg
规格:
20 µg
产品价格:
¥2250.0
规格:
100 µg
产品价格:
¥7880.0
人 FCGRT & B2M Heterodimer蛋白|FCGRT & B2M Heterodimer protein|FCGRT & B2M Heterodimer(Human, C-AVI & His)(产品说明)
蛋白名称:FCGRT & B2M Heterodimer蛋白, FCGRT & B2M Heterodimer protein
蛋白构建:A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (NP_004098.1) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (NP_004039.1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the human FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Human, Recombinant (AVI Tag), Biotinylated (Cat: CT071-H27H-B) bound to Herceptin was 0.03 μM.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Ala 24 & Ile 21
蛋白分子量:The recombinant heterodimer of human FCGRT/B2M comprises 399 (300+99) amino acids and has a calculated molecular mass of 45.3 (33.6+11.7) KDa.
蛋白NP号:NP_004098.1 & NP_004039.1
蛋白氨基酸序列:Met1-Ser297 & Met1-Met119
蛋白标签:C-AVI & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.