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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human CD122 / IL-2RB Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/10.00 µg/20.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥24000.0 |
|---|---|---|---|
| 规格: | 10.00 µg | 产品价格: | ¥2050.0 |
| 规格: | 20.00 µg | 产品价格: | ¥3220.0 |
| 规格: | 100.00 µg | 产品价格: | ¥5170.0 |
蛋白名称:Human CD122 / IL-2RB Protein (His Tag)
蛋白构建:A DNA sequence encoding the human IL2RB (NP_000869.1) (Met1-Asp239) was expressed with a polyhistidine tag at the C-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:Measured by its ability to inhibit IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4 ng/mL of recombinant human IL-15. The ED50 for this effect is typically 2-10 μg/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Ala 27
蛋白分子量:The recombinant human IL2RB consists of 224 amino acids and predicts a molecular mass of 26 kDa.
蛋白NP号:NP_000869.1
蛋白氨基酸序列:Met1-Asp239
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Kong, X; et al. SHR-1916: A Novel PEGylated Interleukin-2 Analogue with Altered Cellular Selectivity and Improved Pharmacokinetic Profiles for Cancer Immunotherapy. Drug design, development and therapy, PMID: 40026336
机体免疫应答过程的复杂性和严格的调控性,是由多种免疫细胞和免疫分子共同参与完成的。而T淋巴细胞的活化,在免疫应答中扮演着相当重要的角色。研究表明,诱导T细胞的活化与增殖需要两种信号:一种是 TCR/CD3 与抗原提呈细胞(APCs)表面特异的 MHCⅡ 抗原肽复合物结合产生的特异性抗原刺激信号;另一种是非特异性协同刺激信号,由 APCs 表面的协同刺激分子和 T 细胞的相应受体相互作用后产生,其中 CD28/B7 是最为重要的协同刺激分子,能增加 IL-2 的产生,加速T细胞增殖,阻止细胞进入
Buffer 1 with Incomplete Medium in the procedures below.] 1) Dilute the cells to approx. 1 x 107 CD3 T cells/ml in Buffer 1. For the standard procedure of processing 5 x 108 T cells, dilute the sample in 50-60 ml of Buffer 1. 2)
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
with one of the positive control stimuli, i.e., CEF or anti-CD3 mAb (Step 13). Wells coated with anti-IL-2 coating buffer should contain only blue spots. Wells coated with anti-IFN- coating buffer should contain only red spots. i. Set sensitivity
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