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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human CD122 / IL-2RB Protein (ECD, Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/10.00 µg/20.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥33640.0 |
|---|---|---|---|
| 规格: | 10.00 µg | 产品价格: | ¥2050.0 |
| 规格: | 20.00 µg | 产品价格: | ¥3220.0 |
| 规格: | 100.00 µg | 产品价格: | ¥5170.0 |
蛋白名称:Human CD122 / IL-2RB Protein (ECD, Fc Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met1-Asp239) of human IL2RB (NP_000869.1) was expressed with the C-terminal Fc region of human IgG1.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:1. Loaded CD122 / IL2RB Protein, Human, Recombinant (Fc Tag)(Cat. 10696-H02H) on Pro A Biosensor, can bind IL2, Human, Mutant with an affinity constant of was 0.3μM as determined by Octet RED System. 2. Measured by its ability to inhibit the IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4.0 ng/mL of recombinant human IL-15. The ED50 for this effect is 1.0-3.8 μg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ala 27
蛋白分子量:The recombinant human IL2 Rβ/Fc is a disulfide-linked homodimeric protein generated after removal of the signal peptide. The reduced monomer consists of 451 amino acids and has a predicted molecular mass of 51.3 kDa. As a result of glycosylation, the apparent molecular mass of rh IL2RB/Fc monomer is approximately 60-65 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_000869.1
蛋白氨基酸序列:Met1-Asp239
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Ptacin, JL; et al. A CD25-biased interleukin-2 for autoimmune therapy engineered via a semi-synthetic organism. Communications medicine, PMID: 38532017
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