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- 普利莱
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- 2025年12月24日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20度避光保存
- 保质期：
一年
- 英文名：
Antifade Solution
- 库存：
大量
- 供应商：
普利莱APPLYGEN
- CAS号：
无
- 规格：
5ml/10ml/25ml
| 规格： | 5ml | 产品价格： | ￥100.0 |
|---|---|---|---|
| 规格： | 10ml | 产品价格： | ￥160.0 |
| 规格： | 25ml | 产品价格： | ￥280.0 |
抗荧光衰减封片剂 (Antifade Solution) 货号：C1210
描述：在进行荧光显微镜观察时，本产品可用于对荧光组织和细胞样品的封片。抗荧光衰减封片剂
以高纯度甘油为介质，内含抗荧光淬灭剂，有强烈的抗荧光衰减作用。样品封片后 4℃或－20℃避
光保存 2-3 周，可最大限度延长荧光持续时间和维持荧光发光强度，同时封片剂中的其它成分有助
于保持组织抗原抗体处于结合状态。适用于所有光谱范围的荧光，如 FITC，Cy2, Cy3, Rhodamine,
Texas Red, Cy5, Cy7，DAPI, AMCA, R6G, Hoechst 33258 和 33342。
包装与保存：
5 ml 或 10 ml 包装，每一 24 x 24 mm 盖玻片用量约为 20-40µl。自然温度运输，收到后－20℃
避光保存至少 1 年。4℃可保存 3 月。
适用范围：
1. 组织细胞免疫荧光组织化学染色样品封片；
2. 染色体荧光观察封片；
3. 核酸荧光原位杂交样品封片；
对所有光谱范围的荧光均有抗衰减作用，如 FITC，Cy2, Rhodamine, Hoechst 33258 和 33342 等。
使用方法：
1. 吸去载玻片上的液体，略微晾干残留液体。
2. 取 20-40µl 封片剂滴加在载玻片样品处，也可直接加到细胞培养孔内观察。
3. 盖上盖玻片。盖玻片规格为 24 x 24 mm 或 24 x 60 mm 大小。
4. 轻轻压盖玻片，用滤纸小心吸除盖玻片周围多余液体，室温放置数分钟。
5. 直接进行荧光显微镜观察。玻片 4℃或－20℃避光保存 2 周荧光无明显衰减。
6. 如需永久封片可用市售指甲油封固盖玻片周围，但通常这样做并无必要。
注意事项：
1.封片溶液成分无特殊毒性。
2.如盖玻片脱落或错位，可将玻片放入 PBS 中后取出盖玻片，重新用封片剂封片。
3.可将适量抗荧光衰减封片剂直接加到细胞培养孔内，进行荧光观察。
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文献和实验该产品被引用文献
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相关实验
DAPI Counterstaining Protocols
实验试剂 Choose one of the following forms of DAPI: DAPI dihydrochloride (MW = 350.3) DAPI dihydrochloride, FluoroPure™ grade (=98% pure) DAPI dilactate (MW = 457.5) For fluorescence microscopy
may take some time to completely dissolve in water and sonication may be necessary. Note: Neither of these DAPI derivatives is very soluble in phosphate-buffered saline (PBS). For long-term storage the stock solution can be aliquoted and stored at ≤–20°C
for each d12 mm coverslip, 25 µl for square coverslip and 50µl for full size coverslip• Seal the coverslip with nail polish• Store in 4 C up to 2 weeksDAPI stock• 4’, 6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI) from Sigma (Cat# D-9542), 1 mg 1mg
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抗荧光衰减封片剂(不含DAPI) C1210 厂家直销，提供OEM定制服务，大包装更优惠
￥100 - 280
