- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E12637r
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Methionine Adenosyltransferase I,MAT1A
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Methionine Adenosyltransferase I, MAT1A ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MAT1A,MAT; MATA1; SAMS; SAMS1; MAT 1; MAT-I/III; S-adenosylmethionine synthase isoform type-1; S-adenosylmethionine synthetase isoform type-1; adoMet synthase 1; adoMet synthetase 1; methionine adenosyltransferase 1; methionine adenosyltransferase I/III; methionine adenosyltransferase I alpha
Search name
Rat MAT1A ELISA KIT ,Rat MAT ELISA KIT ,Rat MATA1 ELISA KIT ,Rat SAMS ELISA KIT ,Rat SAMS1 ELISA KIT ,Rat MAT 1 ELISA KIT ,Rat MAT-I/III ELISA KIT ,Rat S-adenosylmethionine synthase isoform type-1 ELISA KIT ,Rat S-adenosylmethionine synthetase isoform type-1 ELISA KIT ,Rat adoMet synthase 1 ELISA KIT ,Rat adoMet synthetase 1 ELISA KIT ,Rat methionine adenosyltransferase 1 ELISA KIT ,Rat methionine adenosyltransferase I/III ELISA KIT ,Rat methionine adenosyltransferase I alpha ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat s-adenosylmethionine synthase isoform type-1,AdoMet synthase 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to AdoMet synthase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AdoMet synthase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AdoMet synthase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AdoMet synthase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Analysis of Oxidative Modification of Proteins
, nitrotyrosine (when nitrogen species are the oxidants), and isoaspartate. Methods described in this unit include spectrophotometry, immunoblotting, radiolabeling, GC/MS, ELISA adapted for analysis of oxidative modification.
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