- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E1535r
- 2025年10月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat NLR family CARD domain-containing protein 4,NLRC4 ELISA KI
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat NLR family CARD domain- containing protein 4, NLRC4 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
NLRC4,AIFEC; CARD12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; IPAF; CARD, Clan protein, LRR and NACHT-containing protein; ICE-protease activating factor; NLR family CARD domain-containing protein 4; NOD-like receptor C4; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4; NLR family CARD domain containing 4
Search name
Rat NLRC4 ELISA KIT,Rat AIFEC ELISA KIT,Rat CARD12 ELISA KIT,Rat CLAN ELISA KIT,Rat CLAN1 ELISA KIT,Rat CLANA ELISA KIT,Rat CLANB ELISA KIT,Rat CLANC ELISA KIT,Rat CLAND ELISA KIT,Rat CLR2.1 ELISA KIT,Rat FCAS4 ELISA KIT,Rat IPAF ELISA KIT,Rat CARD ELISA KIT, Rat Clan protein ELISA KIT,Rat LRR and NACHT-containing protein ELISA KIT,Rat ICE-protease activating factor ELISA KIT,Rat NLR family CARD domain-containing protein 4 ELISA KIT,Rat NOD-like receptor C4 ELISA KIT,Rat caspase recruitment domain family member 12 ELISA KIT,Rat caspase recruitment domain-containing protein 12 ELISA KIT,Rat nucleotide-binding oligomerization domain ELISA KIT,Rat leucine rich repeat and CARD domain containing 4 ELISA KIT,Rat NLR family CARD domain containing 4 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat NLR family CARD domain-containing protein 4, NLRC4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NLRC4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NLRC4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NLRC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NLRC4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Immunity：厦门大学韩家淮团队揭示 PELO 控制 NOD 样受体家族蛋白寡聚化组装和激活的机制
(Nucleotide-binding and oligomerization domain, NOD 或 NACHT) ；除 NLRP10 外，所有 NLR 蛋白的 C 端都含有亮氨酸重复序列 (leucine-rich repeat, LRR)，而其 N 末端则由各异的蛋白相互作用结构域构成，如 CARD、PYD 结构域等【3】。大量研究表明，依赖 NACHT 结构域介导的寡聚化是 NLR 蛋白激活的共同特征和主要方式；但目前人们对 NLR 家族蛋白寡聚化组装激活的机制并不清楚。 2023 年 3 月 21
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
/ Cold shock domain-containing proteins 213. DAtA http://luggagefast.Stanford.EDU/group/arabprotein/ Annotated coding sequences from Arabidopsis 214. DExH/D Family Database http://www.helicase.net/dexhd/dbhome.htm DEAD-box, DEAH-box and DExH-box
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