- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0399r
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat High mobility group protein B1,HMGB1
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat High mobility group protein B1, HMGB1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
High mobility group protein B1,;High mobility group protein 1(HMG-1),; HMGB1,high mobility group protein B1; HMG1; HMG3; SBP-1;
Amphoterin; HMG-1; Sulfoglucuronyl carbohydrate binding protein; high mobility group protein 1; high-mobility group (nonhistone
chromosomal) protein 1; high-mobility group box 1; high mobility group box 1
Search name
Rat High mobility group protein B1 ELISA Kit, Rat HMGB1 ELISA Kit, Rat High mobility group protein 1 ELISA Kit, Rat HMG-1
ELISA Kit, Rat HMG3 ELISA Kit, Rat SBP-1 ELISA Kit, Rat Amphoterin ELISA Kit, Rat HMG-1 ELISA Kit, Rat Sulfoglucuronyl
carbohydrate binding protein ELISA Kit, Rat nonhistone chromosomal protein 1 ELISA Kit, Rat high-mobility group box 1 ELISA
Kit, Rat high mobility group box 1 ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat High mobility group protein B1 concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to High mobility group protein B1. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for High
mobility group protein B1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then
a TMB substrate solution is added to each well. Only those wells that contain High mobility group protein B1, biotin-conjugated antibody
and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric
acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of High
mobility group protein B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验High-Mobility Group Box-1 Isoforms as Potential Therapeutic Targets in Sepsis
High-mobility group box-1 (HMGB1) protein was originally described as a nuclear DNA-binding protein that functions as a structural cofactor critical for proper transcriptional regulation and gene expression. Recent studies
Fluorescent Biosensors for the Detection of HMGB1 Release
During necrosis and following some instances of apoptosis (in particular in the absence of a proficient phagocytic system), the nonhistone chromatin component high-mobility group box 1 (HMGB1) is released in the extracellular space. In vivo
对肝纤维化的发生、发展发挥重要作用呢？我们课题组的先前研究表明在肝纤维大鼠的肝组织中HMGB1的表达明显增强，但HMGB1是否通过激活HSC参与肝纤维化的发生发展目前尚不清楚，本研究拟通过HMGB1刺激HSC以观察ECM的合成情况来进一步探讨HMGB1与肝纤维化的关系。 1 材料与方法 1.1 主要试剂 HMGB1由本室制备保存；新生胎牛血清（FCS）为杭州四季青生物工程材料研究所产品；高糖型DMEM购自英国Gibco公司；大鼠PⅢP ，CⅣ双抗体夹心ELISA检测试剂盒购自
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