- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-49129r
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Autophagy protein 5,Atg5
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Autophagy protein 5, ATG5 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ATG5, Autophagy protein 5,ATG5 autophagy related 5 homolog; APG5; APG5-LIKE; APG5L; ASP; hAPG5; apoptosis-specific protein; autophagy related 5
Search name
Rat ATG5 ELISA KIT ,Rat Autophagy protein 5 ELISA KIT ,Rat ATG5 autophagy related 5 homolog ELISA KIT ,Rat APG5 ELISA KIT ,Rat APG5-LIKE ELISA KIT ,Rat APG5L ELISA KIT ,Rat ASP ELISA KIT ,Rat hAPG5 ELISA KIT ,Rat apoptosis-specific protein ELISA KIT ,Rat autophagy related 5 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Autophagy protein 5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy protein 5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Autophagy protein 5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Autophagy protein 5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Autophagy protein 5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Examining Ubiquitinated Protein Aggregates in Tissue Sections
In the cell, the binding of ubiquitin to abnormal or misfolded proteins marks them for degradation by the proteasome or lysosome via autophagy. Ubiquitinated-protein aggregates form when an increase in protein misfolding exceeds
cathy12303 有没有哪位前辈用过cAMP的ELISA试剂盒？ dianaofyezi 我 有问题可以站内消息或者QQ52187644联系。 白大褂2009 你好，我是新手，你用过的ELISA试剂在哪买的，哪个公司的比较好啊？还有，测定大鼠脑组织里的cAMP，放免法和ELISA法各有什么优缺点啊？谢谢回复！！ songdianwei 我在 R&D公司买的，测
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