Rat Platelet- activating facto

Rat Platelet- activating factor acetylhydrolase IB subunit alpha, PAFAH1B1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PAFAH1B1,PAFAHA,LIS1; LIS2; MDCR; MDS; PAFAH; LIS-1; PAF acetylhydrolase 45 kDa subunit; PAF-AH 45 kDa subunit; PAF-AH alpha; PAFAH alpha; lissencephaly 1 protein; lissencephaly-1 protein; platelet-activating factor acetylhydrolase IB subunit alpha; platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kD; platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 45kDa; platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 45kDa

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Platelet- activating factor acetylhydrolase IB subunit alpha, PAFAH1B1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PAFAH1B1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PAFAH1B1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PAFAH1B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PAFAH1B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.