- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-44615r
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Neurofilament light polypeptide,Nefl
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Neurofilament light polypeptide, NEFL ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY!
Alternative name
Rat NEFL ELISA KIT ,Rat Neurofilament light polypeptide ELISA KIT ,Rat NF-L ELISA KIT ,Rat CMT1F ELISA KIT ,Rat CMT2E ELISA KIT ,Rat NF-L ELISA KIT ,Rat NF68 ELISA KIT ,Rat NFL ELISA KIT ,Rat PPP1R110 ELISA KIT ,Rat light molecular weight neurofilament protein ELISA KIT ,Rat neurofilament protein light chain ELISA KIT ,Rat neurofilament subunit NF-L ELISA KIT ,Rat neurofilament triplet L protein ELISA KIT ,Rat neurofilament light polypeptide 68kDa ELISA KIT ,Rat protein phosphatase 1 regulatory subunit 110 ELISA KIT ,Rat neurofilament light polypeptide ELISA KIT ,Rat neurofilament L ELISA KIT ,Rat 68 kDa neurofilament protein ELISA KIT ,Rat Neurofilament triplet L protein ELISA KIT
Intended use
Rat NEFL ELISA KIT allows for the in vitro quantitative determination of rat neurofilament light polypeptide concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Rat NEFL ELISA KIT has been pre-coated with an antibody specific to NEFL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NEFL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NEFL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NEFL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Rat NEFL ELISA KIT Range 15.6-1000pg/ml
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
l/well of 1:625 Biotinylated Goat anti-rat IgG prepared by adding 32 µl IgG (Calbiochem #60653) to 20 ml blocking buffer with the addition of 200 µl of 10% Tween. Return plate to the 4°C on the rotator for 6 hours.• APPLYING STREPTAVIDINAfter 6 hours
Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
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