Rat 6- phosphofructo- 2- kinas

Rat 6- phosphofructo- 2- kinase/fructose- 2,6- biphosphatase 3, PFKFB3 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3,6PF-2-K/Fru-2, 6-P2ase 3,iPFK-2;6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme;Renal carcinoma antigen NY-REN-56,PFKFB3,6 phosphofructo 2 kinase fructose 2 6 bisphosphatase,IPFK2; PFK2; 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase; 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; 6PF-2-K/Fru-2,6-P2ase 3; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; PFK/FBPase 3; fructose-6-phosphate,2-kinase/fructose-2, 6-bisphosphatase; iPFK-2; inducible 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase; renal carcinoma antigen NY-REN-56; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3

Search name

Rat 6PF-2-K ELISA KIT,Rat Fru-2, 6-P2ase 3 ELISA KIT,Rat 6PF-2-K/Fru-2 ELISA KIT,Rat PFKFB3 ELISA KIT,Rat iPFK-2 ELISA KIT,Rat IPFK2 ELISA KIT,Rat iPFK-2 ELISA KIT,Rat 6-P2ase brain/placenta-type isozyme ELISA KIT,Rat Renal carcinoma antigen NY-REN-56 ELISA KIT,Rat 6 phosphofructo 2 kinase fructose 2 6 bisphosphatase ELISA KIT,Rat PFK2, 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase ELISA KIT,Rat  6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase ELISA KIT,Rat 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 ELISA KIT,

Rat 6PF-2-K/Fru-2,6-P2ase 3 ELISA KIT,Rat 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme, PFK/FBPase 3 ELISA KIT,Rat fructose-6-phosphate,2-kinase/fructose-2, 6-bisphosphatase ELISA KIT,Rat inducible 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase ELISA KIT,Rat renal carcinoma antigen NY-REN-56 ELISA KIT,Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3, 6PF-2-K concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to 6PF-2-K. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for 6PF-2-K and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 6PF-2-K, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 6PF-2-K in the samples is then determined by comparing the O.D. of the samples to the standard curve.